A method for detecting candida species in a specimen by polymerase chain reaction.

• Main Authors: Chong, Pei Pei, Mohammed Harmal, Nabil Saad, Jamal, Farida, Sekawi, Zamberi
• Format: Patent
• Language: English
• Published: 2011
• Online Access: http://psasir.upm.edu.my/id/eprint/32082/

The present invention relates to a method for detecting Candida species in a specimen, comprising the steps of extracting nucleic acid from the specimen; performing polymerase chain reaction on the extracted nucleic acid using DNA primers for amplification of targeted nucleic acid sequence; detecting the amplified nucleic acid for each Candida species; characterized in that, in a single reaction, the DNA primers consist of: SEQ ID NO. 1 and SEQ ID NO. 2 or their complements for amplifying nucleic acid from Candida albicans; SEQ ID NO.3 and SEQ ID NO. 4 or their complements for amplifying nucleic acid from Candida glabrata; SEQ ID NO. 5 and SEQ ID NO. 6 or their complements for amplifying nucleic acid from Candida parapsilosis; SEQ ID NO. 7 and SEQ ID NO. 8 or their complements for amplifying nucleic acid from Candida tropicalis.