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Mucosal genetic immunization through microsphere-based oral carriers.

Polymeric carriers in the form of cellulose acetate phthalate (CAP) and alginate (ALG) microspheres were used for encapsulation of plasmid DNA for oral mucosal immunization. Access into the intestinal mucosa by pVAXI eukaryotic expression plasmid vectors carrying gene-coding sequences, either for th...

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Main Authors: Rosli, Rozita, Nograles, Nadine, Hanafi, Aimi Melati, Shamsudin, Mariana Nor, Abdullah, Syahrilnizam
Format: Article
Language:English
Published: Landes Bioscience 2013
Online Access:http://psasir.upm.edu.my/id/eprint/29817/1/Mucosal%20genetic%20immunization%20through%20microsphere.pdf
http://psasir.upm.edu.my/id/eprint/29817/
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spelling my.upm.eprints.298172015-10-29T08:15:56Z http://psasir.upm.edu.my/id/eprint/29817/ Mucosal genetic immunization through microsphere-based oral carriers. Rosli, Rozita Nograles, Nadine Hanafi, Aimi Melati Shamsudin, Mariana Nor Abdullah, Syahrilnizam Polymeric carriers in the form of cellulose acetate phthalate (CAP) and alginate (ALG) microspheres were used for encapsulation of plasmid DNA for oral mucosal immunization. Access into the intestinal mucosa by pVAXI eukaryotic expression plasmid vectors carrying gene-coding sequences, either for the cholera enterotoxin B subunit (cfxB) immunostimulatory antigen or the green fluorescent protein (GFP), delivered from both types of microsphere carriers were examined in orally immunized BALB/c mice. Demonstration of transgene protein expression and IgA antibody responses at local mucosal sites suggest immunological response to a potential oral DNA vaccine formulated within the microsphere carriers. Landes Bioscience 2013-10 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29817/1/Mucosal%20genetic%20immunization%20through%20microsphere.pdf Rosli, Rozita and Nograles, Nadine and Hanafi, Aimi Melati and Shamsudin, Mariana Nor and Abdullah, Syahrilnizam (2013) Mucosal genetic immunization through microsphere-based oral carriers. Human Vaccines and Immunotherapeutics, 9 (10). pp. 2222-2227. ISSN 2164-5515; ESSN: 2164-554X 10.4161/hv.25325
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Polymeric carriers in the form of cellulose acetate phthalate (CAP) and alginate (ALG) microspheres were used for encapsulation of plasmid DNA for oral mucosal immunization. Access into the intestinal mucosa by pVAXI eukaryotic expression plasmid vectors carrying gene-coding sequences, either for the cholera enterotoxin B subunit (cfxB) immunostimulatory antigen or the green fluorescent protein (GFP), delivered from both types of microsphere carriers were examined in orally immunized BALB/c mice. Demonstration of transgene protein expression and IgA antibody responses at local mucosal sites suggest immunological response to a potential oral DNA vaccine formulated within the microsphere carriers.
format Article
author Rosli, Rozita
Nograles, Nadine
Hanafi, Aimi Melati
Shamsudin, Mariana Nor
Abdullah, Syahrilnizam
spellingShingle Rosli, Rozita
Nograles, Nadine
Hanafi, Aimi Melati
Shamsudin, Mariana Nor
Abdullah, Syahrilnizam
Mucosal genetic immunization through microsphere-based oral carriers.
author_facet Rosli, Rozita
Nograles, Nadine
Hanafi, Aimi Melati
Shamsudin, Mariana Nor
Abdullah, Syahrilnizam
author_sort Rosli, Rozita
title Mucosal genetic immunization through microsphere-based oral carriers.
title_short Mucosal genetic immunization through microsphere-based oral carriers.
title_full Mucosal genetic immunization through microsphere-based oral carriers.
title_fullStr Mucosal genetic immunization through microsphere-based oral carriers.
title_full_unstemmed Mucosal genetic immunization through microsphere-based oral carriers.
title_sort mucosal genetic immunization through microsphere-based oral carriers.
publisher Landes Bioscience
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/29817/1/Mucosal%20genetic%20immunization%20through%20microsphere.pdf
http://psasir.upm.edu.my/id/eprint/29817/
_version_ 1643829873663279104
score 13.18916

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