Candida albicans isolates from a Malaysian hospital exhibit more potent phospholipase and haemolysin activities than non-albicans Candida isolates
This study was aimed at determining the phospholipase and haemolysin activity of Candida isolates in Malaysia. A total of 37 Candida clinical isolates representing seven species, Candida albicans (12), Candida tropicalis (8), Candida glabrata (4), Candida parapsilosis (1), Candida krusei (4), Candid...
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Malaysian Society of Parasitology and Tropical Medicine
2013
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Online Access: | http://psasir.upm.edu.my/id/eprint/29693/1/Candida%20albicans%20isolates%20from%20a%20Malaysian%20hospital%20exhibit%20more%20potent%20phospholipase%20and%20haemolysin%20activities%20than%20non.pdf http://psasir.upm.edu.my/id/eprint/29693/ http://www.msptm.org/files/654_-_662_Chong_PP.pdf |
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my.upm.eprints.296932015-09-08T01:42:34Z http://psasir.upm.edu.my/id/eprint/29693/ Candida albicans isolates from a Malaysian hospital exhibit more potent phospholipase and haemolysin activities than non-albicans Candida isolates Chin, Voon Kin Foong, Kuan Jeang Abdullah, Maha Basir, Rusliza Mohtarrudin, Norhafizah Ng, Kee Peng Chong, Pei Pei This study was aimed at determining the phospholipase and haemolysin activity of Candida isolates in Malaysia. A total of 37 Candida clinical isolates representing seven species, Candida albicans (12), Candida tropicalis (8), Candida glabrata (4), Candida parapsilosis (1), Candida krusei (4), Candida orthopsilosis (1) and Candida rugosa (7) were tested. In vitro phospholipase activity was determined by using egg yolk plate assay whereas in vitro haemolysin activity was tested by using blood plate assay on sheep blood Sabouraud's dextrose agar (SDA) enriched with glucose. Phospholipase activity was detected in 75% (9 out of 12) of the C. albicans isolates. Among the 25 non- C. albicans Candida isolates, phospholipase activity was detected in only 24% of these isolates. The phospholipase activity of C. albicans was significantly higher than that of the non- C. albicans Candida isolates (P=0.002). Haemolysin activity was detected in 100% of the C. albicans, C. tropicalis, C. glabrata, C. krusei, C. parapsilosis, and C. orthopsilosis isolates while 75% of the C. krusei isolates and 12.3% of the C. rugosa isolates showed haemolysin activity. The haemolytic activity of C. albicans was significantly higher than that of the non- C. albicans Candida isolates (P=0.0001).The findings in this study indicate that C. albicans isolates in Malaysia may possess greater virulence potential than the non-albicans species. Malaysian Society of Parasitology and Tropical Medicine 2013-12 Article NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29693/1/Candida%20albicans%20isolates%20from%20a%20Malaysian%20hospital%20exhibit%20more%20potent%20phospholipase%20and%20haemolysin%20activities%20than%20non.pdf Chin, Voon Kin and Foong, Kuan Jeang and Abdullah, Maha and Basir, Rusliza and Mohtarrudin, Norhafizah and Ng, Kee Peng and Chong, Pei Pei (2013) Candida albicans isolates from a Malaysian hospital exhibit more potent phospholipase and haemolysin activities than non-albicans Candida isolates. Tropical Biomedicine, 30 (4). pp. 654-662. ISSN 0127-5720 http://www.msptm.org/files/654_-_662_Chong_PP.pdf |
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This study was aimed at determining the phospholipase and haemolysin activity of Candida isolates in Malaysia. A total of 37 Candida clinical isolates representing seven species, Candida albicans (12), Candida tropicalis (8), Candida glabrata (4), Candida parapsilosis (1), Candida krusei (4), Candida orthopsilosis (1) and Candida rugosa (7) were tested. In vitro phospholipase activity was determined by using egg yolk plate assay whereas in vitro haemolysin activity was tested by using blood plate assay on sheep blood Sabouraud's dextrose agar (SDA) enriched with glucose. Phospholipase activity was detected in 75% (9 out of 12) of the C. albicans isolates. Among the 25 non- C. albicans Candida isolates, phospholipase activity was detected in only 24% of these isolates. The phospholipase activity of C. albicans was significantly higher than that of the non- C. albicans Candida isolates (P=0.002). Haemolysin activity was detected in 100% of the C. albicans, C. tropicalis, C. glabrata, C. krusei, C. parapsilosis, and C. orthopsilosis isolates while 75% of the C. krusei isolates and 12.3% of the C. rugosa isolates showed haemolysin activity. The haemolytic activity of C. albicans was significantly higher than that of the non- C. albicans Candida isolates (P=0.0001).The findings in this study indicate that C. albicans isolates in Malaysia may possess greater virulence potential than the non-albicans species. |
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Article |
author |
Chin, Voon Kin Foong, Kuan Jeang Abdullah, Maha Basir, Rusliza Mohtarrudin, Norhafizah Ng, Kee Peng Chong, Pei Pei |
spellingShingle |
Chin, Voon Kin Foong, Kuan Jeang Abdullah, Maha Basir, Rusliza Mohtarrudin, Norhafizah Ng, Kee Peng Chong, Pei Pei Candida albicans isolates from a Malaysian hospital exhibit more potent phospholipase and haemolysin activities than non-albicans Candida isolates |
author_facet |
Chin, Voon Kin Foong, Kuan Jeang Abdullah, Maha Basir, Rusliza Mohtarrudin, Norhafizah Ng, Kee Peng Chong, Pei Pei |
author_sort |
Chin, Voon Kin |
title |
Candida albicans isolates from a Malaysian hospital exhibit more potent phospholipase and haemolysin activities than non-albicans Candida isolates |
title_short |
Candida albicans isolates from a Malaysian hospital exhibit more potent phospholipase and haemolysin activities than non-albicans Candida isolates |
title_full |
Candida albicans isolates from a Malaysian hospital exhibit more potent phospholipase and haemolysin activities than non-albicans Candida isolates |
title_fullStr |
Candida albicans isolates from a Malaysian hospital exhibit more potent phospholipase and haemolysin activities than non-albicans Candida isolates |
title_full_unstemmed |
Candida albicans isolates from a Malaysian hospital exhibit more potent phospholipase and haemolysin activities than non-albicans Candida isolates |
title_sort |
candida albicans isolates from a malaysian hospital exhibit more potent phospholipase and haemolysin activities than non-albicans candida isolates |
publisher |
Malaysian Society of Parasitology and Tropical Medicine |
publishDate |
2013 |
url |
http://psasir.upm.edu.my/id/eprint/29693/1/Candida%20albicans%20isolates%20from%20a%20Malaysian%20hospital%20exhibit%20more%20potent%20phospholipase%20and%20haemolysin%20activities%20than%20non.pdf http://psasir.upm.edu.my/id/eprint/29693/ http://www.msptm.org/files/654_-_662_Chong_PP.pdf |
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13.18916 |