Evaluation of PCR-based approach for serotype determination of Streptococcus pneumoniae
Determination of Streptococcus pneumoniae serotypes is essential for epidemiological surveillance. Therefore accurate, reliable and cost effective serotyping method is crucial. In this study, we determined the serotypes of 41 pneumococcal isolates recovered from human anterior nares by multiplex Pol...
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Malaysian Society of Parasitology and Tropical Medicine
2013
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Online Access: | http://psasir.upm.edu.my/id/eprint/29586/1/Evaluation%20of%20PCR-based%20approach%20for%20serotype%20determination%20of%20Streptococcus%20pneumoniae.pdf http://psasir.upm.edu.my/id/eprint/29586/ http://www.msptm.org/journal26.html |
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my.upm.eprints.295862016-08-03T03:36:28Z http://psasir.upm.edu.my/id/eprint/29586/ Evaluation of PCR-based approach for serotype determination of Streptococcus pneumoniae Mohamed Shakrin, Nik Noorul Shakira Balasubramaniam, Shandra Devi Ahmad Yusof @ Hanafi, Hanani Mastuki, Mohd Fahmi Masri, Siti Norbaya Mohd Taib, Niazlin Amin Nordin, Syafinaz Jamal, Farida Fatema @ Farida Clarke, Stuart C. Mohd Desa, Mohd Nasir Determination of Streptococcus pneumoniae serotypes is essential for epidemiological surveillance. Therefore accurate, reliable and cost effective serotyping method is crucial. In this study, we determined the serotypes of 41 pneumococcal isolates recovered from human anterior nares by multiplex Polymerase Chain Reaction (PCR) utilizing published primers. The data was then compared with conventional serology using latex agglutination (LA) and the Quellung reaction. Based on the PCR-approach, 8 different serogroups/serotypes were detected with one isolate classified as non-typeable (cpsA- negative). In reference to the serology-based data, the results were in agreement except for one isolate. For the latter isolate, the LA and Quellung tests failed to show a reaction but the PCR-approach and sequencing identified the isolate as serogroup 15B/C. Based on this experimental setting, we found that the PCR-approach for pneumococcal serotypes determination is reliable to serve as the alternative for determining the pneumococcal serotyping. Malaysian Society of Parasitology and Tropical Medicine 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29586/1/Evaluation%20of%20PCR-based%20approach%20for%20serotype%20determination%20of%20Streptococcus%20pneumoniae.pdf Mohamed Shakrin, Nik Noorul Shakira and Balasubramaniam, Shandra Devi and Ahmad Yusof @ Hanafi, Hanani and Mastuki, Mohd Fahmi and Masri, Siti Norbaya and Mohd Taib, Niazlin and Amin Nordin, Syafinaz and Jamal, Farida Fatema @ Farida and Clarke, Stuart C. and Mohd Desa, Mohd Nasir (2013) Evaluation of PCR-based approach for serotype determination of Streptococcus pneumoniae. Tropical Biomedicine, 30 (2). pp. 338-344. ISSN 0127-5720 http://www.msptm.org/journal26.html |
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Determination of Streptococcus pneumoniae serotypes is essential for epidemiological surveillance. Therefore accurate, reliable and cost effective serotyping method is crucial. In this study, we determined the serotypes of 41 pneumococcal isolates recovered from human anterior nares by multiplex Polymerase Chain Reaction (PCR) utilizing published primers. The data was then compared with conventional serology using latex agglutination (LA) and the Quellung reaction. Based on the PCR-approach, 8 different serogroups/serotypes were detected with one isolate classified as non-typeable (cpsA- negative). In reference to the serology-based data, the results were in agreement except for one isolate. For the latter isolate, the LA and Quellung tests failed to show a reaction but the PCR-approach and sequencing identified the isolate as serogroup 15B/C. Based on this experimental setting, we found that the PCR-approach for pneumococcal serotypes determination is reliable to serve as the alternative for determining the pneumococcal serotyping. |
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Article |
author |
Mohamed Shakrin, Nik Noorul Shakira Balasubramaniam, Shandra Devi Ahmad Yusof @ Hanafi, Hanani Mastuki, Mohd Fahmi Masri, Siti Norbaya Mohd Taib, Niazlin Amin Nordin, Syafinaz Jamal, Farida Fatema @ Farida Clarke, Stuart C. Mohd Desa, Mohd Nasir |
spellingShingle |
Mohamed Shakrin, Nik Noorul Shakira Balasubramaniam, Shandra Devi Ahmad Yusof @ Hanafi, Hanani Mastuki, Mohd Fahmi Masri, Siti Norbaya Mohd Taib, Niazlin Amin Nordin, Syafinaz Jamal, Farida Fatema @ Farida Clarke, Stuart C. Mohd Desa, Mohd Nasir Evaluation of PCR-based approach for serotype determination of Streptococcus pneumoniae |
author_facet |
Mohamed Shakrin, Nik Noorul Shakira Balasubramaniam, Shandra Devi Ahmad Yusof @ Hanafi, Hanani Mastuki, Mohd Fahmi Masri, Siti Norbaya Mohd Taib, Niazlin Amin Nordin, Syafinaz Jamal, Farida Fatema @ Farida Clarke, Stuart C. Mohd Desa, Mohd Nasir |
author_sort |
Mohamed Shakrin, Nik Noorul Shakira |
title |
Evaluation of PCR-based approach for serotype determination of Streptococcus pneumoniae |
title_short |
Evaluation of PCR-based approach for serotype determination of Streptococcus pneumoniae |
title_full |
Evaluation of PCR-based approach for serotype determination of Streptococcus pneumoniae |
title_fullStr |
Evaluation of PCR-based approach for serotype determination of Streptococcus pneumoniae |
title_full_unstemmed |
Evaluation of PCR-based approach for serotype determination of Streptococcus pneumoniae |
title_sort |
evaluation of pcr-based approach for serotype determination of streptococcus pneumoniae |
publisher |
Malaysian Society of Parasitology and Tropical Medicine |
publishDate |
2013 |
url |
http://psasir.upm.edu.my/id/eprint/29586/1/Evaluation%20of%20PCR-based%20approach%20for%20serotype%20determination%20of%20Streptococcus%20pneumoniae.pdf http://psasir.upm.edu.my/id/eprint/29586/ http://www.msptm.org/journal26.html |
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1643829805010911232 |
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13.18916 |