Effect of Carbon and Nitrogen Sources on the Growth and Production of Cellulase Enzymes of a Newly Isolated Aspergillus sp.

The culture filtrate obtained from a newly isolated Aspergillus sp. exhibited good activities against the filter paper as substrate. The optimum temperature and pH values for growth were 3'?C and 6.5, respectively. However, the optimum temperature and pH for the activities of the cellulase enzy...

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Main Authors: Tong, Chow Chin, Rajendra, K.
Format: Article
Language:English
English
Published: 1992
Online Access:http://psasir.upm.edu.my/id/eprint/2947/1/Effect_of_Carbon_and_Nitrogen_Sources_on_the_Growth_and_Production.pdf
http://psasir.upm.edu.my/id/eprint/2947/
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spelling my.upm.eprints.29472013-05-27T07:04:38Z http://psasir.upm.edu.my/id/eprint/2947/ Effect of Carbon and Nitrogen Sources on the Growth and Production of Cellulase Enzymes of a Newly Isolated Aspergillus sp. Tong, Chow Chin Rajendra, K. The culture filtrate obtained from a newly isolated Aspergillus sp. exhibited good activities against the filter paper as substrate. The optimum temperature and pH values for growth were 3'?C and 6.5, respectively. However, the optimum temperature and pH for the activities of the cellulase enzymes were recorded as 44'C and 4.5, respectively. Production of the enzymes in liquid medium reached its maximum level (9 units/ml) at the 15th day of incubation with an incubation period of up to 21 days. From the various carbon and nitrogen sources tested, carboxymethyl cellulose (medium viscosity) and (NH) 2 Fe(SO)2' 6H20 were found to be the best for cellulase enzyme production, whereas cellulose powder and NH/103 enhanced mycelial growth. 1992 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/2947/1/Effect_of_Carbon_and_Nitrogen_Sources_on_the_Growth_and_Production.pdf Tong, Chow Chin and Rajendra, K. (1992) Effect of Carbon and Nitrogen Sources on the Growth and Production of Cellulase Enzymes of a Newly Isolated Aspergillus sp. Pertanika, 15 (1). pp. 45-50. English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description The culture filtrate obtained from a newly isolated Aspergillus sp. exhibited good activities against the filter paper as substrate. The optimum temperature and pH values for growth were 3'?C and 6.5, respectively. However, the optimum temperature and pH for the activities of the cellulase enzymes were recorded as 44'C and 4.5, respectively. Production of the enzymes in liquid medium reached its maximum level (9 units/ml) at the 15th day of incubation with an incubation period of up to 21 days. From the various carbon and nitrogen sources tested, carboxymethyl cellulose (medium viscosity) and (NH) 2 Fe(SO)2' 6H20 were found to be the best for cellulase enzyme production, whereas cellulose powder and NH/103 enhanced mycelial growth.
format Article
author Tong, Chow Chin
Rajendra, K.
spellingShingle Tong, Chow Chin
Rajendra, K.
Effect of Carbon and Nitrogen Sources on the Growth and Production of Cellulase Enzymes of a Newly Isolated Aspergillus sp.
author_facet Tong, Chow Chin
Rajendra, K.
author_sort Tong, Chow Chin
title Effect of Carbon and Nitrogen Sources on the Growth and Production of Cellulase Enzymes of a Newly Isolated Aspergillus sp.
title_short Effect of Carbon and Nitrogen Sources on the Growth and Production of Cellulase Enzymes of a Newly Isolated Aspergillus sp.
title_full Effect of Carbon and Nitrogen Sources on the Growth and Production of Cellulase Enzymes of a Newly Isolated Aspergillus sp.
title_fullStr Effect of Carbon and Nitrogen Sources on the Growth and Production of Cellulase Enzymes of a Newly Isolated Aspergillus sp.
title_full_unstemmed Effect of Carbon and Nitrogen Sources on the Growth and Production of Cellulase Enzymes of a Newly Isolated Aspergillus sp.
title_sort effect of carbon and nitrogen sources on the growth and production of cellulase enzymes of a newly isolated aspergillus sp.
publishDate 1992
url http://psasir.upm.edu.my/id/eprint/2947/1/Effect_of_Carbon_and_Nitrogen_Sources_on_the_Growth_and_Production.pdf
http://psasir.upm.edu.my/id/eprint/2947/
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score 13.211869