Proses Penghasilan Bromelin daripada Batang Nenas
A proteolytic enzyme called bromelin can be produced from pineapple stem by precipitating it from pineapple stem juice and separating the precipitate from the juice. Acetone is used as the precipitant. The precipitating process is performed twice and the second precipitate is taken as the bromelin...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English English |
| Published: |
1990
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| Online Access: | http://psasir.upm.edu.my/id/eprint/2885/1/Proses_Penghasilan_Bromelin_daripada_Batang_Nenas.pdf http://psasir.upm.edu.my/id/eprint/2885/ |
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| Summary: | A proteolytic enzyme called bromelin can be produced from pineapple stem by precipitating it from pineapple
stem juice and separating the precipitate from the juice. Acetone is used as the precipitant. The precipitating
process is performed twice and the second precipitate is taken as the bromelin product. This process is not
well understood and is not amenable to mathematical modelling. The main aim of the present study is to
identify process variables such as the precipitant: juice ratio, precipitation time and centrifugation time in
every stage which have a significant effect on the yield and activity of bromelin. Factor analysis is used.
This study found that the centrifugation times in both stages, precipitation and centrifugation times in the
second stage affect the yield of bromelin. The enzyme activity is affected by the precipitant: juice ratio, the
precipitation and centrifugation times in the first stage,' precipitant: juice ratio and precipitation time in
the second stage. This process is currently being optimised. |
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