An effective protein extraction method for two-dimensional electrophoresis in the anticancer herb Andrographis paniculata Nees

• Main Authors: Talei, Daryush, Valdiani, Alireza, Abdullah, Mohd Puad
• Format: Article
• Language: English
• Published: International Union of Biochemistry and Molecular Biology 2013
• Online Access: http://psasir.upm.edu.my/id/eprint/28142/1/An%20effective%20protein%20extraction%20method%20for%20two.pdf; http://psasir.upm.edu.my/id/eprint/28142/

Proteomic analysis of plants relies on high yields of pure protein. In plants, protein extraction and purification present a great challenge due to accumulation of a large amount of interfering substances, including polysaccharides, polyphenols, and secondary metabolites. Therefore, it is necessary...