Integrated acoustic immunoaffinity-capture (IAI) platform for detection of PSA from whole blood samples.

• Main Authors: Ahmad Tajudin, Asilah, Petersson, K., Lenshof, A., Sward-Nilsson, A. M., Aberg, L., Marko-Varga, G., Malm, J., Lilja, H., Laurell, T.
• Format: Article
• Language: English; English
• Published: Royal Society of Chemistry 2013
• Online Access: http://psasir.upm.edu.my/id/eprint/28121/1/Integrated%20acoustic%20immunoaffinity.pdf; http://psasir.upm.edu.my/id/eprint/28121/

On-chip detection of low abundant protein biomarkers is of interest to enable point-of-care diagnostics. Using a simple form of integration, we have realized an integrated microfluidic platform for the detection of prostate specific antigen (PSA), directly in anti-coagulated whole blood. We combine acoustophoresis-based separation of plasma from undiluted whole blood with a miniaturized immunoassay system in a polymer manifold, demonstrating improved assay speed on our Integrated Acoustic Immunoaffinity-capture (IAI) platform. The IAI platform separates plasma from undiluted whole blood by means of acoustophoresis and provides cell free plasma of clinical quality at a rate of 10 uL/min for an online immunoaffinity-capture of PSA on a porous silicon antibody microarray. The whole blood input (hematocrit 38-40%) rate was 50 μl min(-1) giving a plasma volume fraction yield of ≈33%. PSA was immunoaffinity-captured directly from spiked female whole blood samples at clinically significant levels of 1.7-100 ng ml(-1) within 15 min and was subsequently detected via fluorescence readout, showing a linear response over the entire range with a coefficient of variation of 13%.