Construction of single-chain variable fragment antibodies against MCF-7 breast cancer cells.

• Main Authors: Ahmad, Zuhaida Asra, Ali, Abdul Manaf, Subramani, Tamilselvan, Mohamed Alitheen, Noorjahan Banu, Hamid, Muhajir, Noor, A. M., Yeap, Swee Keong
• Format: Article
• Language: English; English
• Published: Fundacao de Pesquisas Cientificas de Ribeirao Preto 2013
• Online Access: http://psasir.upm.edu.my/id/eprint/28118/1/Construction%20of%20single.pdf; http://psasir.upm.edu.my/id/eprint/28118/; http://www.geneticsmr.com/issue/12/4?page=4

A phage display library of single chain variable fragment (scFv) against MCF-7 breast cancer cells was constructed from C3A8 hybridoma cells. RNA from the C3A8 was isolated, cDNA was constructed, and variable heavy and light immunoglobulin chain gene region were amplified using PCR. The variable hea...