Goniothalamin selectively induces apoptosis on human hepatoblastoma cells through caspase-3 activation.

• Main Authors: Al-Qubaisi, Mothanna, Rosli, Rozita, Subramani, Tamilselvan, Omar, Abdul Rahman, Yeap, Swee Keong, Ali, Abdul Manaf, Mohamed Alitheen, Noorjahan Banu
• Format: Article
• Language: English; English
• Published: Taylor & Francis 2013
• Online Access: http://psasir.upm.edu.my/id/eprint/28113/1/Goniothalamin%20selectively%20induces%20apoptosis%20on%20human%20hepatoblastoma%20cells%20through%20caspase.pdf; http://psasir.upm.edu.my/id/eprint/28113/

oniothalamin is a biologically active styrylpyrone derivative isolated from various Goniothalamus species. The ability of goniothalamin to induce apoptosis via caspase-3 activation against hepatoblastoma (HepG2) and normal liver cells (Chang cells) was studied using morphological and biochemical evaluations. HepG2 and Chang cells were treated with goniothalamin for 72 h and analysed by TUNEL and Annexin-V/PI staining. Furthermore, the post-mitochondrial caspase-3 was quantified using ELISA. In view of our results, goniothalamin induced apoptosis on treated cells via alteration of cellular membrane integrity and cleavage of DNA. On the other hand, post-mitochondrial caspase-3 activity was significantly elevated in HepG2 cells treated with goniothalamin after 72 h. These findings suggest that goniothalamin induced apoptosis on HepG2 liver cancer cells via induction of caspase-3 with less sensitivity on the cell line of Chang cells.