Effect of donor cells concentration on colonization of human spermatogonial stem cells in recipient mouse testes.

• Main Authors: Mirzapour, Tooba, Movahedin, Mansoureh, Tengku Ibrahim, Tengku Azmi, Haron, Abdul Wahid, Makoolati , Zohreh, Nowroozi, Mohamadreza
• Format: Article
• Language: English; English
• Published: Asian Network for Scientific Information 2010
• Online Access: http://psasir.upm.edu.my/id/eprint/25580/1/Effect%20of%20donor%20cells%20concentration%20on%20colonization%20of%20human%20spermatogonial%20stem%20cells%20in%20recipient%20mouse%20testes.pdf; http://psasir.upm.edu.my/id/eprint/25580/

Exogenesis (cross-species) germ cell transplantation provides an opportunity to investigate fundamental aspects of spermatogenesis. In this study, testis biopsies of patients with maturation arrest of spermatogenesis during a one year ago were first minced mechanically into small pieces and then Spermatogonial Stem Cells (SSCs) and Sertoli cells isolated by the two- step enzymatic digestion, were plated and grown on DSA-Lectin coated dishes in Dulbecco's Modified Eagle's Medium (DMEM) containing 10% fetal calf serum. Transplantation of human spermatogonial cells into mouse recipient testis was performed on day 7 (before colony formation) and 2 weeks after culturing (colony formation). The effects of different concentrations of spermatogonial cell on quantity of transplantation and percent of colonized seminiferous tubules were assayed during 8 weeks after transplantation. The result showed that SSCs can be observed on the basement membrane of the seminiferous tubules in place of spermatogonial stem cells and proliferation occurs about 4 weeks after transplantation. The difference in donor cells concentration had more effect on colonization of mouse recipient testis (p<0.05). It will be an alternative approach for the repopulation of infertile seminiferous tubules and preservation of fertility, in the future