Improved in vitro development of cloned bovine embryos using S-adenosylhomocysteine, a non-toxic epigenetic modifying reagent.

• Main Authors: Jafari, Shahram, Hosseini, Morteza S., Hajian, Mehdi, Forouzanfar, Mohsen, Jafarpour, Farnoosh, Abedi, Parvaneh, Ostadhosseini, Somayyeh, Abbasi, Hassan, Gourabi, Hamid, Shahverdi, Abdolhossein H., Dizaj, A. V. T., Anjomshoaa, Maryam, Haron, Wahid, Noor, Noorshariza, Yakub, Halimhatoon, Nasr Esfahani, Mohammad Hosein
• Format: Article
• Language: English; English
• Published: Wiley 2011
• Online Access: http://psasir.upm.edu.my/id/eprint/25579/1/Improved%20in%20vitro%20development%20of%20cloned%20bovine%20embryos%20using%20S2.pdf; http://psasir.upm.edu.my/id/eprint/25579/

In this study, fibroblast cells were stably transfected with mouse POU5F1 promoter-driven enhanced green fluorescent protein (EGFP) to investigate the effect of S-adenosylhomocysteine (SAH), the reversible non-toxic inhibitor of DNA-methyltransferases (DNMTs), at different intervals post-fusion on i...