Detection of plasmids in heavy metal resistance bacteria isolated from the Persian Gulf and enclosed industrial areas
Several heavy metal resistant bacterial strains were isolated from sediment and water samples collected from the Persian Gulf and enclosed industrial areas. All the isolated bacteria were identified by 16S rRNA gene sequencing. Isolated bacteria were tested for the presence of plasmids using th...
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National Institute of Genetic Engineering and Biotechnology
2007
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Online Access: | http://psasir.upm.edu.my/id/eprint/25444/1/Detection%20of%20plasmids%20in%20heavy%20metal%20resistance%20bacteria%20isolated%20from%20the%20Persian%20Gulf%20and%20enclosed%20industrial%20areas.pdf http://psasir.upm.edu.my/id/eprint/25444/ http://www.ijbiotech.com/issue_1142_1166_Volume+5%2C+Issue+4%2C+Autumn+2007.html |
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my.upm.eprints.254442016-12-27T02:50:07Z http://psasir.upm.edu.my/id/eprint/25444/ Detection of plasmids in heavy metal resistance bacteria isolated from the Persian Gulf and enclosed industrial areas Zolgharnein, Hossein Mohd Lila, Mohd Azmi Saad, Mohd Zamri Mutalib, Abdul Rahim Mohamed, Che Abd Rahim Several heavy metal resistant bacterial strains were isolated from sediment and water samples collected from the Persian Gulf and enclosed industrial areas. All the isolated bacteria were identified by 16S rRNA gene sequencing. Isolated bacteria were tested for the presence of plasmids using the modified alkaline lysate method. The method was effective for identification and characterization of plasmids of different sizes without the use of highly toxic chemicals. The study revealed that the frequency of the occurrence of plasmids in heavy metal resistant bacteria was more than that in the common bacteria. The study also demonstrated that about 66% of isolated bacteria carried large (38-62kb) and/or small sized (4- >2 kb) plasmids. The highest plasmid incidence (84.6%) was detected from industrial wastewater bacteria. A slightly higher incidence of plasmids occurred in bacteria isolated from marine sediments (55.5%) compared to that of the marine water (53.8%). The findings suggested that plasmids are highly ubiquitous and predominant in most heavy metal resistant bacteria. Removal of lead and cadmium from solution by some of these bacteria was very efficient, approximately 120 mg/g dry weightas high as 90%. The isolates tested, presented distinct uptake capacities and the best results were obtained for Delftia tsuruhatensis and Pseudomonas AU3411 respectively. National Institute of Genetic Engineering and Biotechnology 2007-10 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/25444/1/Detection%20of%20plasmids%20in%20heavy%20metal%20resistance%20bacteria%20isolated%20from%20the%20Persian%20Gulf%20and%20enclosed%20industrial%20areas.pdf Zolgharnein, Hossein and Mohd Lila, Mohd Azmi and Saad, Mohd Zamri and Mutalib, Abdul Rahim and Mohamed, Che Abd Rahim (2007) Detection of plasmids in heavy metal resistance bacteria isolated from the Persian Gulf and enclosed industrial areas. Iranian Journal of Biotechnology, 5 (4). pp. 232-239. ISSN 1728-3043; ESSN: 2322-2921 http://www.ijbiotech.com/issue_1142_1166_Volume+5%2C+Issue+4%2C+Autumn+2007.html |
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Several heavy metal resistant bacterial strains were
isolated from sediment and water samples collected
from the Persian Gulf and enclosed industrial areas.
All the isolated bacteria were identified by 16S rRNA
gene sequencing. Isolated bacteria were tested for the
presence of plasmids using the modified alkaline
lysate method. The method was effective for identification
and characterization of plasmids of different sizes
without the use of highly toxic chemicals. The study
revealed that the frequency of the occurrence of plasmids
in heavy metal resistant bacteria was more than
that in the common bacteria. The study also demonstrated
that about 66% of isolated bacteria carried
large (38-62kb) and/or small sized (4- >2 kb) plasmids.
The highest plasmid incidence (84.6%) was detected
from industrial wastewater bacteria. A slightly higher
incidence of plasmids occurred in bacteria isolated
from marine sediments (55.5%) compared to that of
the marine water (53.8%). The findings suggested that
plasmids are highly ubiquitous and predominant in
most heavy metal resistant bacteria. Removal of lead
and cadmium from solution by some of these bacteria
was very efficient, approximately 120 mg/g dry weightas
high as 90%. The isolates tested, presented distinct
uptake capacities and the best results were obtained
for Delftia tsuruhatensis and Pseudomonas AU3411
respectively. |
format |
Article |
author |
Zolgharnein, Hossein Mohd Lila, Mohd Azmi Saad, Mohd Zamri Mutalib, Abdul Rahim Mohamed, Che Abd Rahim |
spellingShingle |
Zolgharnein, Hossein Mohd Lila, Mohd Azmi Saad, Mohd Zamri Mutalib, Abdul Rahim Mohamed, Che Abd Rahim Detection of plasmids in heavy metal resistance bacteria isolated from the Persian Gulf and enclosed industrial areas |
author_facet |
Zolgharnein, Hossein Mohd Lila, Mohd Azmi Saad, Mohd Zamri Mutalib, Abdul Rahim Mohamed, Che Abd Rahim |
author_sort |
Zolgharnein, Hossein |
title |
Detection of plasmids in heavy metal resistance bacteria
isolated from the Persian Gulf and enclosed industrial
areas |
title_short |
Detection of plasmids in heavy metal resistance bacteria
isolated from the Persian Gulf and enclosed industrial
areas |
title_full |
Detection of plasmids in heavy metal resistance bacteria
isolated from the Persian Gulf and enclosed industrial
areas |
title_fullStr |
Detection of plasmids in heavy metal resistance bacteria
isolated from the Persian Gulf and enclosed industrial
areas |
title_full_unstemmed |
Detection of plasmids in heavy metal resistance bacteria
isolated from the Persian Gulf and enclosed industrial
areas |
title_sort |
detection of plasmids in heavy metal resistance bacteria
isolated from the persian gulf and enclosed industrial
areas |
publisher |
National Institute of Genetic Engineering and Biotechnology |
publishDate |
2007 |
url |
http://psasir.upm.edu.my/id/eprint/25444/1/Detection%20of%20plasmids%20in%20heavy%20metal%20resistance%20bacteria%20isolated%20from%20the%20Persian%20Gulf%20and%20enclosed%20industrial%20areas.pdf http://psasir.upm.edu.my/id/eprint/25444/ http://www.ijbiotech.com/issue_1142_1166_Volume+5%2C+Issue+4%2C+Autumn+2007.html |
_version_ |
1643828660767031296 |
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13.189131 |