Tissue culture isolation, electron microscopic characterization and PCR-detection of a betanodavirus isolated from diseased Asian sea bass.

• Main Authors: F. Hasoon, Mauida, Mohd. Daud, Hassan, Arshad, Siti Suri, Bejo, Mohd Hair
• Format: Article
• Language: English; English
• Published: 2010
• Online Access: http://psasir.upm.edu.my/id/eprint/25412/1/Tissue%20culture%20isolation.pdf; http://psasir.upm.edu.my/id/eprint/25412/

A viral agent was successfully isolated from Asian sea bass juveniles (Letes calcarifer) exhibiting clinical signs of viral nervous necrosis (VNN) in southern Malaysia on February 2008. Eyes and brains of diseased fish were pooled, extracted and filtered. The filtrates were inoculated on SSN-1 cells...