Verification of X- and Y-spermatozoa separation by nested polymerase chain reaction (PCR), motility and membrane integrity in bovine

• Main Authors: Abdul Malik,, Haron, Abd Wahid, Yusoff, Rosnina, Bukar, Muhammad Modu, Kasim, Azhar, Mohd Yusoff, Md Sabri
• Format: Article
• Language: English
• Published: Academic Journals 2011
• Online Access: http://psasir.upm.edu.my/id/eprint/25345/1/25345.pdf; http://psasir.upm.edu.my/id/eprint/25345/; http://www.academicjournals.org/journal/AJB/article-abstract/F158CC438333

The aim of the present study was to verify the presence of X- and Y-chromosome spermatozoa after separation with swimming speed using oestrus cows vagina mucus, Percoll discontinuous gradient (45 to 90%) and swim-up using TALP medium. The nested polymerase chain reaction (PCR) was used to determine...