Biocompatibility of Gentamicin-coated Hydroxyapatite (Ha) with osteoblast.
This study was carried out to compare the surface morphological changes and cell-to-cell attachment with different concentrations of gentamicin-coated hydroxyapatite (HA) on the osteoblast cell lines. Osteoblast cell lines were cultured and maintained in complete medium, 1:1 HAM’s F12 Medium Dul...
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my.upm.eprints.246872014-11-04T08:59:19Z http://psasir.upm.edu.my/id/eprint/24687/ Biocompatibility of Gentamicin-coated Hydroxyapatite (Ha) with osteoblast. Othman, Fauziah Che Seman, Che Nor Zarida Tan, K. S. Au, Lee Fong Moin, Saidi Besar, Idris Mustaffa, Rusnah Rahmat, Asmah Goriman Khan, Mohd Azam Khan Ahmad, Azfar Rizal This study was carried out to compare the surface morphological changes and cell-to-cell attachment with different concentrations of gentamicin-coated hydroxyapatite (HA) on the osteoblast cell lines. Osteoblast cell lines were cultured and maintained in complete medium, 1:1 HAM’s F12 Medium Dulbecco's modified Eagle's medium without phenol red (DMEM) and incubated at 37oC in 5% CO2. The cell lines were treated with gentamicin-coated HA and undergone the 3-(4,5-di-methylthiazol-2-yl)-2,5- diphenyl tetrazolium bromide (MTT) assay. After 72 hours treatment, the gentamicin-coated HA was processed for viewing the morphological changes by using scanning electron microscopy. The MTT assay results indicated that varying concentrations of gentamicin coated hydroxyapatite do not significantly affect viability of osteoblast. The osteoblast-like cells attached and spread well on the surface of hydroxyapatite and grew into the pores. In conclusion, the gentamicin-coated HA is biocompatible towards osteoblast. 2010 Article PeerReviewed Othman, Fauziah and Che Seman, Che Nor Zarida and Tan, K. S. and Au, Lee Fong and Moin, Saidi and Besar, Idris and Mustaffa, Rusnah and Rahmat, Asmah and Goriman Khan, Mohd Azam Khan and Ahmad, Azfar Rizal (2010) Biocompatibility of Gentamicin-coated Hydroxyapatite (Ha) with osteoblast. Medical Journal of Malaysia, 65 (10). pp. 87-89. ISSN 0300-5283 http://www.e-mjm.org/2010/Sup_tissue_engineering_part3.pdf |
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This study was carried out to compare the surface morphological changes and cell-to-cell attachment
with different concentrations of gentamicin-coated
hydroxyapatite (HA) on the osteoblast cell lines.
Osteoblast cell lines were cultured and maintained in
complete medium, 1:1 HAM’s F12 Medium Dulbecco's modified Eagle's medium without phenol red (DMEM) and incubated at 37oC in 5% CO2. The cell lines were treated with gentamicin-coated HA and undergone the 3-(4,5-di-methylthiazol-2-yl)-2,5- diphenyl tetrazolium bromide (MTT) assay. After 72 hours treatment, the gentamicin-coated HA was processed for viewing the morphological changes by using scanning electron microscopy. The MTT assay results indicated that varying concentrations of gentamicin coated hydroxyapatite do not significantly affect viability of osteoblast. The osteoblast-like cells attached and spread well on the surface of hydroxyapatite and grew into the pores. In
conclusion, the gentamicin-coated HA is biocompatible towards osteoblast. |
format |
Article |
author |
Othman, Fauziah Che Seman, Che Nor Zarida Tan, K. S. Au, Lee Fong Moin, Saidi Besar, Idris Mustaffa, Rusnah Rahmat, Asmah Goriman Khan, Mohd Azam Khan Ahmad, Azfar Rizal |
spellingShingle |
Othman, Fauziah Che Seman, Che Nor Zarida Tan, K. S. Au, Lee Fong Moin, Saidi Besar, Idris Mustaffa, Rusnah Rahmat, Asmah Goriman Khan, Mohd Azam Khan Ahmad, Azfar Rizal Biocompatibility of Gentamicin-coated Hydroxyapatite (Ha) with osteoblast. |
author_facet |
Othman, Fauziah Che Seman, Che Nor Zarida Tan, K. S. Au, Lee Fong Moin, Saidi Besar, Idris Mustaffa, Rusnah Rahmat, Asmah Goriman Khan, Mohd Azam Khan Ahmad, Azfar Rizal |
author_sort |
Othman, Fauziah |
title |
Biocompatibility of Gentamicin-coated Hydroxyapatite (Ha) with osteoblast. |
title_short |
Biocompatibility of Gentamicin-coated Hydroxyapatite (Ha) with osteoblast. |
title_full |
Biocompatibility of Gentamicin-coated Hydroxyapatite (Ha) with osteoblast. |
title_fullStr |
Biocompatibility of Gentamicin-coated Hydroxyapatite (Ha) with osteoblast. |
title_full_unstemmed |
Biocompatibility of Gentamicin-coated Hydroxyapatite (Ha) with osteoblast. |
title_sort |
biocompatibility of gentamicin-coated hydroxyapatite (ha) with osteoblast. |
publishDate |
2010 |
url |
http://psasir.upm.edu.my/id/eprint/24687/ http://www.e-mjm.org/2010/Sup_tissue_engineering_part3.pdf |
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1643828435803439104 |
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13.154949 |