Screening method for detection of immediate amino acid decarboxylases - producing bacteria implicated in food poisoning
Assessment of amino acid decarboxylase activity can be conducted using tubed broth or plated agar. In this study, the test was carried out in microtitre plates containing lysine, ornithine, arginine, tyrosine, tryptophan, phenylalanine or histidine as biogenic amine precursors. Møller decarboxylase...
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Malaysian Society of Parasitology and Tropical Medicine
2011
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Online Access: | http://psasir.upm.edu.my/id/eprint/24098/1/24098.pdf http://psasir.upm.edu.my/id/eprint/24098/ http://msptm.org/journal-vol-28-no-2/ |
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my.upm.eprints.240982019-12-18T04:00:50Z http://psasir.upm.edu.my/id/eprint/24098/ Screening method for detection of immediate amino acid decarboxylases - producing bacteria implicated in food poisoning Hussain, Husniza Abd Razak, Mohd Fuat Balasubramaniam, Vimala Mohd Ghazali, Hasanah Assessment of amino acid decarboxylase activity can be conducted using tubed broth or plated agar. In this study, the test was carried out in microtitre plates containing lysine, ornithine, arginine, tyrosine, tryptophan, phenylalanine or histidine as biogenic amine precursors. Møller decarboxylase base broth (MDB) with or without 1% of a known amino acid were added to wells of a 96 well-microtitre plate. The wells were inoculated with Escherichia coli, Klebsiella pneumoniae, Acinetobacter anitratus or Staphylococcus aureus to the final concentration of 6.0 x 10(7) cfu/ml and incubated at 35ºC. The absorbance of the culture broth was read at 570 nm at 0, 1.0, 2.0, 3.0, 4.0, 5.5, 6.5 and 7.5 hour. Comparison of means of A'(570) between 0 hour and a specified incubation time was determined statistically. Positive decarboxylase activities were detected in the media inoculated with E. coli and K. pneumoniae in less than 6 hours. The current method is suitable for immediate producers of amino acid decarboxylase enzymes. It costs less as it uses less amino acid and it has the potential to be used for screening aliquots of food materials for amino acid decarboxylase activities. Malaysian Society of Parasitology and Tropical Medicine 2011 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/24098/1/24098.pdf Hussain, Husniza and Abd Razak, Mohd Fuat and Balasubramaniam, Vimala and Mohd Ghazali, Hasanah (2011) Screening method for detection of immediate amino acid decarboxylases - producing bacteria implicated in food poisoning. Tropical Biomedicine, 28 (2). pp. 351-361. ISSN 0127-5720 http://msptm.org/journal-vol-28-no-2/ |
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Assessment of amino acid decarboxylase activity can be conducted using tubed broth or plated agar. In this study, the test was carried out in microtitre plates containing lysine, ornithine, arginine, tyrosine, tryptophan, phenylalanine or histidine as biogenic amine precursors. Møller decarboxylase base broth (MDB) with or without 1% of a known amino acid were added to wells of a 96 well-microtitre plate. The wells were inoculated with Escherichia coli, Klebsiella pneumoniae, Acinetobacter anitratus or Staphylococcus aureus to the final concentration of 6.0 x 10(7) cfu/ml and incubated at 35ºC. The absorbance of the culture broth was read at 570 nm at 0, 1.0, 2.0, 3.0, 4.0, 5.5, 6.5 and 7.5 hour. Comparison of means of A'(570) between 0 hour and a specified incubation time was determined statistically. Positive decarboxylase activities were detected in the media inoculated with E. coli and K. pneumoniae in less than 6 hours. The current method is suitable for immediate producers of amino acid decarboxylase enzymes. It costs less as it uses less amino acid and it has the potential to be used for screening aliquots of food materials for amino acid decarboxylase activities. |
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Hussain, Husniza Abd Razak, Mohd Fuat Balasubramaniam, Vimala Mohd Ghazali, Hasanah |
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Hussain, Husniza Abd Razak, Mohd Fuat Balasubramaniam, Vimala Mohd Ghazali, Hasanah Screening method for detection of immediate amino acid decarboxylases - producing bacteria implicated in food poisoning |
author_facet |
Hussain, Husniza Abd Razak, Mohd Fuat Balasubramaniam, Vimala Mohd Ghazali, Hasanah |
author_sort |
Hussain, Husniza |
title |
Screening method for detection of immediate amino acid decarboxylases - producing bacteria implicated in food poisoning |
title_short |
Screening method for detection of immediate amino acid decarboxylases - producing bacteria implicated in food poisoning |
title_full |
Screening method for detection of immediate amino acid decarboxylases - producing bacteria implicated in food poisoning |
title_fullStr |
Screening method for detection of immediate amino acid decarboxylases - producing bacteria implicated in food poisoning |
title_full_unstemmed |
Screening method for detection of immediate amino acid decarboxylases - producing bacteria implicated in food poisoning |
title_sort |
screening method for detection of immediate amino acid decarboxylases - producing bacteria implicated in food poisoning |
publisher |
Malaysian Society of Parasitology and Tropical Medicine |
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2011 |
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http://psasir.upm.edu.my/id/eprint/24098/1/24098.pdf http://psasir.upm.edu.my/id/eprint/24098/ http://msptm.org/journal-vol-28-no-2/ |
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