Purification, characterization and thermal inactivation kinetics of a non-regioselective thermostable lipase from a genotypically identified extremophilic Bacillus subtilis NS 8.

• Main Authors: Olusesan, Akanbi Taiwo, Kamaruzaman, Azura Liana, Forghani, Bita, Abu Bakar, Fatimah, Mohammed, Abdulkarim Sabo, Radu, Son, Abd. Manap, Mohd. Yazid, Saari, Nazamid
• Format: Article
• Language: English
• Published: Elsevier 2011
• Online Access: http://psasir.upm.edu.my/id/eprint/24096/

Thermostable lipase produced by a genotypically identified extremophilic Bacillus subtilis NS 8 was purified 500-fold to homogeneity with a recovery of 16% by ultrafiltration, DEAE-Toyopearl 650M and Sephadex G-75 column. The purified enzyme showed a prominent single band with a molecular weight of...