Purification, characterization and thermal inactivation kinetics of a non-regioselective thermostable lipase from a genotypically identified extremophilic Bacillus subtilis NS 8.
Thermostable lipase produced by a genotypically identified extremophilic Bacillus subtilis NS 8 was purified 500-fold to homogeneity with a recovery of 16% by ultrafiltration, DEAE-Toyopearl 650M and Sephadex G-75 column. The purified enzyme showed a prominent single band with a molecular weight of...
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Main Authors: | , , , , , , , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2011
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Online Access: | http://psasir.upm.edu.my/id/eprint/24096/ |
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