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Extraction of microsomal fraction from midguts of western spruce budworm Choristoneura occidentalis F., and rat liver, and their ability to expoxidize aldrin

Microsomal fractions were isolated from midguts of western spruce budworm larvae and rat liver. The microsomal preparations were then assayed for cytochrome P-450 content and aldrin epoxidase activity. Both microsomal preparations showed distinct spectrum of cytochrome P-450. Relative to protein...

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Main Author: Mohammad, Rosli
Format: Article
Language:English
Published: Universiti Pertanian Malaysia 1985
Online Access:http://psasir.upm.edu.my/id/eprint/2366/1/Extraction_of_Microsomal_Fraction_from.pdf
http://psasir.upm.edu.my/id/eprint/2366/
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spelling my.upm.eprints.23662015-03-09T06:32:23Z http://psasir.upm.edu.my/id/eprint/2366/ Extraction of microsomal fraction from midguts of western spruce budworm Choristoneura occidentalis F., and rat liver, and their ability to expoxidize aldrin Mohammad, Rosli Microsomal fractions were isolated from midguts of western spruce budworm larvae and rat liver. The microsomal preparations were then assayed for cytochrome P-450 content and aldrin epoxidase activity. Both microsomal preparations showed distinct spectrum of cytochrome P-450. Relative to protein content, the cytochrome P-450 content of midguts of western spruce budworm was lower by 7 - 9 times compared to that of the rat liver. Epoxidase activity is present in the two microsomal preparations as indicated by their ability to convert aldrin to dieldrin. The activity of both microsomal preparations was inhibited by piperonyl butoxide. Universiti Pertanian Malaysia 1985 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/2366/1/Extraction_of_Microsomal_Fraction_from.pdf Mohammad, Rosli (1985) Extraction of microsomal fraction from midguts of western spruce budworm Choristoneura occidentalis F., and rat liver, and their ability to expoxidize aldrin. Pertanika, 8 (2). pp. 231-236. ISSN 0126-6128
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Microsomal fractions were isolated from midguts of western spruce budworm larvae and rat liver. The microsomal preparations were then assayed for cytochrome P-450 content and aldrin epoxidase activity. Both microsomal preparations showed distinct spectrum of cytochrome P-450. Relative to protein content, the cytochrome P-450 content of midguts of western spruce budworm was lower by 7 - 9 times compared to that of the rat liver. Epoxidase activity is present in the two microsomal preparations as indicated by their ability to convert aldrin to dieldrin. The activity of both microsomal preparations was inhibited by piperonyl butoxide.
format Article
author Mohammad, Rosli
spellingShingle Mohammad, Rosli
Extraction of microsomal fraction from midguts of western spruce budworm Choristoneura occidentalis F., and rat liver, and their ability to expoxidize aldrin
author_facet Mohammad, Rosli
author_sort Mohammad, Rosli
title Extraction of microsomal fraction from midguts of western spruce budworm Choristoneura occidentalis F., and rat liver, and their ability to expoxidize aldrin
title_short Extraction of microsomal fraction from midguts of western spruce budworm Choristoneura occidentalis F., and rat liver, and their ability to expoxidize aldrin
title_full Extraction of microsomal fraction from midguts of western spruce budworm Choristoneura occidentalis F., and rat liver, and their ability to expoxidize aldrin
title_fullStr Extraction of microsomal fraction from midguts of western spruce budworm Choristoneura occidentalis F., and rat liver, and their ability to expoxidize aldrin
title_full_unstemmed Extraction of microsomal fraction from midguts of western spruce budworm Choristoneura occidentalis F., and rat liver, and their ability to expoxidize aldrin
title_sort extraction of microsomal fraction from midguts of western spruce budworm choristoneura occidentalis f., and rat liver, and their ability to expoxidize aldrin
publisher Universiti Pertanian Malaysia
publishDate 1985
url http://psasir.upm.edu.my/id/eprint/2366/1/Extraction_of_Microsomal_Fraction_from.pdf
http://psasir.upm.edu.my/id/eprint/2366/
_version_ 1643822297183682560
score 13.189132

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