Genetic variation studies in Oryctes rhinoceros (L.) (Coleoptera: Scarabaeidae) from oil palm plantations using random amplified microsatellite (RAMs) markers
Randomly amplified microsatellite markers were used to study the genetic variation among six populations of Oryctes rhinoceros L. which were collected from oil palm plantations in Selangor, Perak, Pahang and Medan. Samples were collected using light and pheromone trapping for the purpose of obtain...
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2011
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my.upm.eprints.236542017-11-10T02:08:24Z http://psasir.upm.edu.my/id/eprint/23654/ Genetic variation studies in Oryctes rhinoceros (L.) (Coleoptera: Scarabaeidae) from oil palm plantations using random amplified microsatellite (RAMs) markers Gnanasegaram, Manjeri Muhamad Awang, Rita Qamaruz Zaman, Faridah Tan, Soon Guan Randomly amplified microsatellite markers were used to study the genetic variation among six populations of Oryctes rhinoceros L. which were collected from oil palm plantations in Selangor, Perak, Pahang and Medan. Samples were collected using light and pheromone trapping for the purpose of obtaining two populations per site study. Thirty in dividual beetles per population were screened using seven randomly amplified microsatellite primers. Beetles were not attracted to light traps at Pahang and Medan. This resulted in only pheromone populations being caught there. Distances calculated based on the similarity coefficient of Nei and Li (1979) ranged between 0.422 and 0.736. Seventy eight reproducible loci were generated using the seven pr imers and all the loci were polymorphic. The dendrogram constructed produced two major clusters. Based on the dendrogram, the clusterings were observed to be influenced by preference to trapping system as well as geographical distance. The separation of clustering between Perak Pheromone (PP) and Perak Light (PL) is important as it gives rise to the possibility for the presence of two groups of O. rhinoceros based on their preference toward light and pheromone trap. However, further studies using codominant markers especially single locus DNA microsatellite markers are required to understand the population genetic structure and to further validate the presence of a cryptic species complex. Academic Journals 2011-04-04 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/23654/1/23654.pdf Gnanasegaram, Manjeri and Muhamad Awang, Rita and Qamaruz Zaman, Faridah and Tan, Soon Guan (2011) Genetic variation studies in Oryctes rhinoceros (L.) (Coleoptera: Scarabaeidae) from oil palm plantations using random amplified microsatellite (RAMs) markers. African Journal of Biotechnology, 10 (14). art. no. 1A00EBE24786. pp. 2611-2617. ISSN 1684–5315 http://academicjournals.org/journal/AJB/article-abstract/1A00EBE24786 10.5897/AJB10.1537 |
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Randomly amplified microsatellite markers were used
to study the genetic variation among six populations of
Oryctes rhinoceros L. which were collected from oil palm plantations in Selangor, Perak, Pahang and Medan. Samples were collected using light and pheromone trapping for the purpose of obtaining two populations per site study. Thirty in dividual beetles per population were screened using
seven randomly amplified microsatellite primers. Beetles were not attracted to light traps at Pahang and Medan. This resulted in only pheromone populations being caught there. Distances calculated based on the similarity coefficient of Nei and Li (1979) ranged between 0.422 and 0.736. Seventy eight reproducible loci were generated using the seven pr
imers and all the loci were polymorphic. The dendrogram constructed produced two major clusters. Based on the dendrogram, the clusterings were observed to be influenced by preference to trapping system as well as geographical distance. The separation of clustering between Perak Pheromone (PP) and Perak Light (PL) is important as it gives
rise to the possibility for the presence of two groups of
O. rhinoceros based on their preference toward light and pheromone trap. However, further studies using codominant markers especially single locus DNA microsatellite markers are required to understand the population genetic structure and to further validate the presence of a cryptic species complex. |
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Gnanasegaram, Manjeri Muhamad Awang, Rita Qamaruz Zaman, Faridah Tan, Soon Guan |
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Gnanasegaram, Manjeri Muhamad Awang, Rita Qamaruz Zaman, Faridah Tan, Soon Guan Genetic variation studies in Oryctes rhinoceros (L.) (Coleoptera: Scarabaeidae) from oil palm plantations using random amplified microsatellite (RAMs) markers |
author_facet |
Gnanasegaram, Manjeri Muhamad Awang, Rita Qamaruz Zaman, Faridah Tan, Soon Guan |
author_sort |
Gnanasegaram, Manjeri |
title |
Genetic variation studies in Oryctes rhinoceros (L.) (Coleoptera: Scarabaeidae) from oil palm plantations using random amplified microsatellite (RAMs) markers |
title_short |
Genetic variation studies in Oryctes rhinoceros (L.) (Coleoptera: Scarabaeidae) from oil palm plantations using random amplified microsatellite (RAMs) markers |
title_full |
Genetic variation studies in Oryctes rhinoceros (L.) (Coleoptera: Scarabaeidae) from oil palm plantations using random amplified microsatellite (RAMs) markers |
title_fullStr |
Genetic variation studies in Oryctes rhinoceros (L.) (Coleoptera: Scarabaeidae) from oil palm plantations using random amplified microsatellite (RAMs) markers |
title_full_unstemmed |
Genetic variation studies in Oryctes rhinoceros (L.) (Coleoptera: Scarabaeidae) from oil palm plantations using random amplified microsatellite (RAMs) markers |
title_sort |
genetic variation studies in oryctes rhinoceros (l.) (coleoptera: scarabaeidae) from oil palm plantations using random amplified microsatellite (rams) markers |
publisher |
Academic Journals |
publishDate |
2011 |
url |
http://psasir.upm.edu.my/id/eprint/23654/1/23654.pdf http://psasir.upm.edu.my/id/eprint/23654/ http://academicjournals.org/journal/AJB/article-abstract/1A00EBE24786 |
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