Direct recovery of lipase derived from Burkholderia cepacia in recycling aqueous two-phase flotation
In this paper, aqueous two-phase flotation (ATPF) composed of thermo-sensitive ethylene oxide–propylene oxide (EOPO) copolymer and ammonium sulfate was developed for direct recovery of Burkholderia cepacia (B. cepacia) strains ST8 lipase from fermentation broth. The effect of varying polymer molar m...
Saved in:
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2011
|
Online Access: | http://psasir.upm.edu.my/id/eprint/23007/1/Direct%20recovery%20of%20lipase%20derived%20from%20Burkholderia%20cepacia%20in%20recycling%20aqueous%20two-phase%20flotation.pdf http://psasir.upm.edu.my/id/eprint/23007/ https://www.sciencedirect.com/science/article/pii/S1383586611003510#! |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
my.upm.eprints.23007 |
---|---|
record_format |
eprints |
spelling |
my.upm.eprints.230072020-04-15T16:11:23Z http://psasir.upm.edu.my/id/eprint/23007/ Direct recovery of lipase derived from Burkholderia cepacia in recycling aqueous two-phase flotation Show, Pau Loke Tan, Chin Ping Anuar, Mohd Shamsul Ariff, Arbakariya Yusof, Yus Aniza Chen, Soo Kien Ling, Tau Chuan In this paper, aqueous two-phase flotation (ATPF) composed of thermo-sensitive ethylene oxide–propylene oxide (EOPO) copolymer and ammonium sulfate was developed for direct recovery of Burkholderia cepacia (B. cepacia) strains ST8 lipase from fermentation broth. The effect of varying polymer molar mass, concentration of ammonium sulfate, pH, amount of loaded crude feedstock, initial volume of EOPO phase, concentration of EOPO, initial volume of aqueous phase, nitrogen flow rate and flotation time upon ATPF performance were investigated. Under the optimal conditions of ATPF, the average separation efficiency and purification fold are 76% and 13%, respectively. The recycling of phase components was introduced to minimize the use of organic solvent and salt in ATPF. It was demonstrated EOPO phase in the ATPF system was recovered up to 75%. There was no significant difference in selectivity, purification fold, separation efficiency and recovery yield of lipase obtained between ATPF using fresh and recycled chemicals. B. cepacia lipase was successfully purified by using ATPF, which is composed of copolymer EOPO/ammonium sulfate in a single downstream processing step. Elsevier 2011 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/23007/1/Direct%20recovery%20of%20lipase%20derived%20from%20Burkholderia%20cepacia%20in%20recycling%20aqueous%20two-phase%20flotation.pdf Show, Pau Loke and Tan, Chin Ping and Anuar, Mohd Shamsul and Ariff, Arbakariya and Yusof, Yus Aniza and Chen, Soo Kien and Ling, Tau Chuan (2011) Direct recovery of lipase derived from Burkholderia cepacia in recycling aqueous two-phase flotation. Separation and Purification Technology, 80 (3). pp. 577-584. ISSN 1383-5866; ESSN: 1873-3794 https://www.sciencedirect.com/science/article/pii/S1383586611003510#! 10.1016/j.seppur.2011.06.013 |
institution |
Universiti Putra Malaysia |
building |
UPM Library |
collection |
Institutional Repository |
continent |
Asia |
country |
Malaysia |
content_provider |
Universiti Putra Malaysia |
content_source |
UPM Institutional Repository |
url_provider |
http://psasir.upm.edu.my/ |
language |
English |
description |
In this paper, aqueous two-phase flotation (ATPF) composed of thermo-sensitive ethylene oxide–propylene oxide (EOPO) copolymer and ammonium sulfate was developed for direct recovery of Burkholderia cepacia (B. cepacia) strains ST8 lipase from fermentation broth. The effect of varying polymer molar mass, concentration of ammonium sulfate, pH, amount of loaded crude feedstock, initial volume of EOPO phase, concentration of EOPO, initial volume of aqueous phase, nitrogen flow rate and flotation time upon ATPF performance were investigated. Under the optimal conditions of ATPF, the average separation efficiency and purification fold are 76% and 13%, respectively. The recycling of phase components was introduced to minimize the use of organic solvent and salt in ATPF. It was demonstrated EOPO phase in the ATPF system was recovered up to 75%. There was no significant difference in selectivity, purification fold, separation efficiency and recovery yield of lipase obtained between ATPF using fresh and recycled chemicals. B. cepacia lipase was successfully purified by using ATPF, which is composed of copolymer EOPO/ammonium sulfate in a single downstream processing step. |
format |
Article |
author |
Show, Pau Loke Tan, Chin Ping Anuar, Mohd Shamsul Ariff, Arbakariya Yusof, Yus Aniza Chen, Soo Kien Ling, Tau Chuan |
spellingShingle |
Show, Pau Loke Tan, Chin Ping Anuar, Mohd Shamsul Ariff, Arbakariya Yusof, Yus Aniza Chen, Soo Kien Ling, Tau Chuan Direct recovery of lipase derived from Burkholderia cepacia in recycling aqueous two-phase flotation |
author_facet |
Show, Pau Loke Tan, Chin Ping Anuar, Mohd Shamsul Ariff, Arbakariya Yusof, Yus Aniza Chen, Soo Kien Ling, Tau Chuan |
author_sort |
Show, Pau Loke |
title |
Direct recovery of lipase derived from Burkholderia cepacia in recycling aqueous two-phase flotation |
title_short |
Direct recovery of lipase derived from Burkholderia cepacia in recycling aqueous two-phase flotation |
title_full |
Direct recovery of lipase derived from Burkholderia cepacia in recycling aqueous two-phase flotation |
title_fullStr |
Direct recovery of lipase derived from Burkholderia cepacia in recycling aqueous two-phase flotation |
title_full_unstemmed |
Direct recovery of lipase derived from Burkholderia cepacia in recycling aqueous two-phase flotation |
title_sort |
direct recovery of lipase derived from burkholderia cepacia in recycling aqueous two-phase flotation |
publisher |
Elsevier |
publishDate |
2011 |
url |
http://psasir.upm.edu.my/id/eprint/23007/1/Direct%20recovery%20of%20lipase%20derived%20from%20Burkholderia%20cepacia%20in%20recycling%20aqueous%20two-phase%20flotation.pdf http://psasir.upm.edu.my/id/eprint/23007/ https://www.sciencedirect.com/science/article/pii/S1383586611003510#! |
_version_ |
1665895934270111744 |
score |
13.160551 |