Characterization of membrane-bound lipase from a thermophilic Rhizopus oryzae isolated from palm oil mill effluent
The characteristics of the membrane-bound lipase from a thermophilic Rhizopus oryzae were studied. The pH and temperature optima for lipase activity were at 7.0 and 37°C, respectively. The enzyme was stable and acidic conditions, retaining more than 80% of its initial activity at pH 4.0 after 30 min...
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1999
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Online Access: | http://psasir.upm.edu.my/id/eprint/22569/1/Characterization%20of%20membrane-bound%20lipase%20from%20a%20thermophilic%20Rhizopus%20oryzae%20isolated%20from%20palm%20oil%20mill%20effluent.pdf http://psasir.upm.edu.my/id/eprint/22569/7/J%20Americ%20Oil%20Chem%20Soc%20-%201999%20-%20Razak%20-%20Characterization%20of%20membrane%E2%80%90bound%20lipase%20from%20a%20thermophilic%20Rhizopus%20oryzae.pdf http://psasir.upm.edu.my/id/eprint/22569/ http://link.springer.com/article/10.1007%2Fs11746-999-0214-0 |
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my.upm.eprints.225692024-08-06T00:08:08Z http://psasir.upm.edu.my/id/eprint/22569/ Characterization of membrane-bound lipase from a thermophilic Rhizopus oryzae isolated from palm oil mill effluent Abdul Razak, Che Nyonya Musani, Rosiah Basri, Mahiran Salleh, Abu Bakar The characteristics of the membrane-bound lipase from a thermophilic Rhizopus oryzae were studied. The pH and temperature optima for lipase activity were at 7.0 and 37°C, respectively. The enzyme was stable and acidic conditions, retaining more than 80% of its initial activity at pH 4.0 after 30 min incubation. It was stable up to 50°C with 70% of initial activity retained after 3 h incubation. The enzyme is 1,3 specific and exhibits substrate preference. Monoacid triglyceride substrates were hydrolyzed better than methyl esters, polyoxysorbitan and sorbitan substrates. AOCS Press 1999 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/22569/1/Characterization%20of%20membrane-bound%20lipase%20from%20a%20thermophilic%20Rhizopus%20oryzae%20isolated%20from%20palm%20oil%20mill%20effluent.pdf text en http://psasir.upm.edu.my/id/eprint/22569/7/J%20Americ%20Oil%20Chem%20Soc%20-%201999%20-%20Razak%20-%20Characterization%20of%20membrane%E2%80%90bound%20lipase%20from%20a%20thermophilic%20Rhizopus%20oryzae.pdf Abdul Razak, Che Nyonya and Musani, Rosiah and Basri, Mahiran and Salleh, Abu Bakar (1999) Characterization of membrane-bound lipase from a thermophilic Rhizopus oryzae isolated from palm oil mill effluent. Journal of the American Oil Chemists' Society, 76 (2). pp. 171-174. ISSN 0003-021X; ESSN: 1558-9331 http://link.springer.com/article/10.1007%2Fs11746-999-0214-0 10.1007/s11746-999-0214-0 |
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The characteristics of the membrane-bound lipase from a thermophilic Rhizopus oryzae were studied. The pH and temperature optima for lipase activity were at 7.0 and 37°C, respectively. The enzyme was stable and acidic conditions, retaining more than 80% of its initial activity at pH 4.0 after 30 min incubation. It was stable up to 50°C with 70% of initial activity retained after 3 h incubation. The enzyme is 1,3 specific and exhibits substrate preference. Monoacid triglyceride substrates were hydrolyzed better than methyl esters, polyoxysorbitan and sorbitan substrates. |
format |
Article |
author |
Abdul Razak, Che Nyonya Musani, Rosiah Basri, Mahiran Salleh, Abu Bakar |
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Abdul Razak, Che Nyonya Musani, Rosiah Basri, Mahiran Salleh, Abu Bakar Characterization of membrane-bound lipase from a thermophilic Rhizopus oryzae isolated from palm oil mill effluent |
author_facet |
Abdul Razak, Che Nyonya Musani, Rosiah Basri, Mahiran Salleh, Abu Bakar |
author_sort |
Abdul Razak, Che Nyonya |
title |
Characterization of membrane-bound lipase from a thermophilic Rhizopus oryzae isolated from palm oil mill effluent |
title_short |
Characterization of membrane-bound lipase from a thermophilic Rhizopus oryzae isolated from palm oil mill effluent |
title_full |
Characterization of membrane-bound lipase from a thermophilic Rhizopus oryzae isolated from palm oil mill effluent |
title_fullStr |
Characterization of membrane-bound lipase from a thermophilic Rhizopus oryzae isolated from palm oil mill effluent |
title_full_unstemmed |
Characterization of membrane-bound lipase from a thermophilic Rhizopus oryzae isolated from palm oil mill effluent |
title_sort |
characterization of membrane-bound lipase from a thermophilic rhizopus oryzae isolated from palm oil mill effluent |
publisher |
AOCS Press |
publishDate |
1999 |
url |
http://psasir.upm.edu.my/id/eprint/22569/1/Characterization%20of%20membrane-bound%20lipase%20from%20a%20thermophilic%20Rhizopus%20oryzae%20isolated%20from%20palm%20oil%20mill%20effluent.pdf http://psasir.upm.edu.my/id/eprint/22569/7/J%20Americ%20Oil%20Chem%20Soc%20-%201999%20-%20Razak%20-%20Characterization%20of%20membrane%E2%80%90bound%20lipase%20from%20a%20thermophilic%20Rhizopus%20oryzae.pdf http://psasir.upm.edu.my/id/eprint/22569/ http://link.springer.com/article/10.1007%2Fs11746-999-0214-0 |
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