Basic characteristics of cellulase immobilized on lignophenol
Enzymatic hydrolysis of cellulosic biomass by cellulase has been receiving attention. Since the cost of cellulase has a big impact on the price of the products, it is important to develop cheaper alternatives. Here we report on the production of a novel immobilized cellulase. A functional lignin-bas...
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Nihon Kobunshi Gakkai,Society of Polymer Science, Japan
2011
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my.upm.eprints.223492015-12-10T03:36:37Z http://psasir.upm.edu.my/id/eprint/22349/ Basic characteristics of cellulase immobilized on lignophenol Nonaka, Hiroshi Ariffin, Hidayah Funaoka, Masamitsu Enzymatic hydrolysis of cellulosic biomass by cellulase has been receiving attention. Since the cost of cellulase has a big impact on the price of the products, it is important to develop cheaper alternatives. Here we report on the production of a novel immobilized cellulase. A functional lignin-based phenolic polymer “lignophenol” (p-cresol type : ligno-p-cresol) was synthesized from Hinoki wood meal and p-cresol through the phase separation system developed for selective separation of lignocellulosic components. Commercial cellulase derived from Trichoderma reeseiwas easily immobilized on lignophenol simply by mixing to give water-insoluble cellulase-lignophenol complex. Immobilization reached more than 100 mg/g-lignophenol with an enzymatic activity of about 60% compared to free cellulase. With lower immobilization loads, cellulase exhibited lower activity. Cellulase did not significantly detach from lignophenol by mixing with acetate buffer, indicating that the cellulase immobilized by a simple physical adsorption is stable. Nihon Kobunshi Gakkai,Society of Polymer Science, Japan 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22349/1/Basic%20characteristics%20of%20cellulase%20immobilized%20on%20lignophenol.pdf Nonaka, Hiroshi and Ariffin, Hidayah and Funaoka, Masamitsu (2011) Basic characteristics of cellulase immobilized on lignophenol. Kobunshi Ronbunshu, 68 (5). pp. 315-319. ISSN 0386-2186 10.1295/koron.68.315 |
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Enzymatic hydrolysis of cellulosic biomass by cellulase has been receiving attention. Since the cost of cellulase has a big impact on the price of the products, it is important to develop cheaper alternatives. Here we report on the production of a novel immobilized cellulase. A functional lignin-based phenolic polymer “lignophenol” (p-cresol type : ligno-p-cresol) was synthesized from Hinoki wood meal and p-cresol through the phase separation system developed for selective separation of lignocellulosic components. Commercial cellulase derived from Trichoderma reeseiwas easily immobilized on lignophenol simply by mixing to give water-insoluble cellulase-lignophenol complex. Immobilization reached more than 100 mg/g-lignophenol with an enzymatic activity of about 60% compared to free cellulase. With lower immobilization loads, cellulase exhibited lower activity. Cellulase did not significantly detach from lignophenol by mixing with acetate buffer, indicating that the cellulase immobilized by a simple physical adsorption is stable. |
format |
Article |
author |
Nonaka, Hiroshi Ariffin, Hidayah Funaoka, Masamitsu |
spellingShingle |
Nonaka, Hiroshi Ariffin, Hidayah Funaoka, Masamitsu Basic characteristics of cellulase immobilized on lignophenol |
author_facet |
Nonaka, Hiroshi Ariffin, Hidayah Funaoka, Masamitsu |
author_sort |
Nonaka, Hiroshi |
title |
Basic characteristics of cellulase immobilized on lignophenol |
title_short |
Basic characteristics of cellulase immobilized on lignophenol |
title_full |
Basic characteristics of cellulase immobilized on lignophenol |
title_fullStr |
Basic characteristics of cellulase immobilized on lignophenol |
title_full_unstemmed |
Basic characteristics of cellulase immobilized on lignophenol |
title_sort |
basic characteristics of cellulase immobilized on lignophenol |
publisher |
Nihon Kobunshi Gakkai,Society of Polymer Science, Japan |
publishDate |
2011 |
url |
http://psasir.upm.edu.my/id/eprint/22349/1/Basic%20characteristics%20of%20cellulase%20immobilized%20on%20lignophenol.pdf http://psasir.upm.edu.my/id/eprint/22349/ |
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13.18916 |