Basic characteristics of cellulase immobilized on lignophenol

• Main Authors: Nonaka, Hiroshi, Ariffin, Hidayah, Funaoka, Masamitsu
• Format: Article
• Language: English
• Published: Nihon Kobunshi Gakkai,Society of Polymer Science, Japan 2011
• Online Access: http://psasir.upm.edu.my/id/eprint/22349/1/Basic%20characteristics%20of%20cellulase%20immobilized%20on%20lignophenol.pdf; http://psasir.upm.edu.my/id/eprint/22349/

Enzymatic hydrolysis of cellulosic biomass by cellulase has been receiving attention. Since the cost of cellulase has a big impact on the price of the products, it is important to develop cheaper alternatives. Here we report on the production of a novel immobilized cellulase. A functional lignin-based phenolic polymer “lignophenol” (p-cresol type : ligno-p-cresol) was synthesized from Hinoki wood meal and p-cresol through the phase separation system developed for selective separation of lignocellulosic components. Commercial cellulase derived from Trichoderma reeseiwas easily immobilized on lignophenol simply by mixing to give water-insoluble cellulase-lignophenol complex. Immobilization reached more than 100 mg/g-lignophenol with an enzymatic activity of about 60% compared to free cellulase. With lower immobilization loads, cellulase exhibited lower activity. Cellulase did not significantly detach from lignophenol by mixing with acetate buffer, indicating that the cellulase immobilized by a simple physical adsorption is stable.