Enhanced production of periplasmic interferon alpha-2b by Escherichia coli using ion-exchange resin for in situ removal of acetate in the culture

• Main Authors: Tan, Joo Shun, Ramanan, Ramakrishnan Nagasundara, Ling, Tau Chuan, Mustafa, Shuhaimi, Ariff, Arbakariya
• Format: Article
• Language: English
• Published: Elsevier 2011
• Online Access: http://psasir.upm.edu.my/id/eprint/22327/1/Enhanced%20production%20of%20periplasmic%20interferon%20alpha.pdf; http://psasir.upm.edu.my/id/eprint/22327/

The possibility of using in situ addition of anion-exchange resin for the removal of acetate in the culture aimed at improving growth of E. coli and expression of periplasmic human interferon-α2b (PrIFN-α2b) was studied in shake flask culture and stirred tank bioreactor. Different types of anion-exc...