Nanoparticle sensor for label free detection of swine DNA in mixed biological samples

• Main Authors: Ali, Eaqub, Hashim, Uda, Mustafa, Shuhaimi, Che Man, Yaakob, Yusop, Mohd Hazim, Bari, Md. Fazlul, Islam, Kh. Nurul, Hasan, M. F.
• Format: Article
• Language: English
• Published: IOP Publishing 2011
• Online Access: http://psasir.upm.edu.my/id/eprint/22268/1/Nanoparticle%20sensor%20for%20label%20free%20detection%20of%20swine%20DNA%20in%20mixed%20biological%20samples.pdf; http://psasir.upm.edu.my/id/eprint/22268/; http://iopscience.iop.org/article/10.1088/0957-4484/22/19/195503/meta;jsessionid=6FB4B035B384A6724959BDC78A24D781.ip-10-40-2-120

We used 40 ± 5 nm gold nanoparticles (GNPs) as colorimetric sensor to visually detect swine-specific conserved sequence and nucleotide mismatch in PCR-amplified and non-amplified mitochondrial DNA mixtures to authenticate species. Colloidal GNPs changed color from pinkish-red to gray-purple in 2 mM...