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Detection and distinguishing infectious bursal disease virus (IBDV) strains by molecular biology method

The present invention relates to a novel method to detect and differentiate different strains of infectious bursal disease virus (IBDV) in a chicken and other bird sample. RNA was obtained from said samples by using a pair of primer (Primer FVVC & RVVC) in a reverse transriptase-polymerase chain...

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Main Authors: Omar, Abdul Rahman, Bejo, Mohd Hair, Ideris, Aini, Hamzah, Hairul Aini
Format: Patent
Published: 2010
Online Access:http://psasir.upm.edu.my/id/eprint/20452/
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spelling my.upm.eprints.204522015-04-28T12:51:49Z http://psasir.upm.edu.my/id/eprint/20452/ Detection and distinguishing infectious bursal disease virus (IBDV) strains by molecular biology method Omar, Abdul Rahman Bejo, Mohd Hair Ideris, Aini Hamzah, Hairul Aini The present invention relates to a novel method to detect and differentiate different strains of infectious bursal disease virus (IBDV) in a chicken and other bird sample. RNA was obtained from said samples by using a pair of primer (Primer FVVC & RVVC) in a reverse transriptase-polymerase chain reaction. Two different primer combinations (Primer IF & IVIR) and (Primer IF & RCLA) and real-time polymerase chain reaction conditions were designed and optimized for rapid differentiation of very virulent and vaccine strains of IBDV based on detection of signatory threshold cycle (Ct) and melting temperature (Tm) values. 2010-12-28 Patent NonPeerReviewed Abdul Rahman Omar (2010) Detection and distinguishing infectious bursal disease virus (IBDV) strains by molecular biology method. 12/551,969.
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description The present invention relates to a novel method to detect and differentiate different strains of infectious bursal disease virus (IBDV) in a chicken and other bird sample. RNA was obtained from said samples by using a pair of primer (Primer FVVC & RVVC) in a reverse transriptase-polymerase chain reaction. Two different primer combinations (Primer IF & IVIR) and (Primer IF & RCLA) and real-time polymerase chain reaction conditions were designed and optimized for rapid differentiation of very virulent and vaccine strains of IBDV based on detection of signatory threshold cycle (Ct) and melting temperature (Tm) values.
format Patent
author Omar, Abdul Rahman
Bejo, Mohd Hair
Ideris, Aini
Hamzah, Hairul Aini
spellingShingle Omar, Abdul Rahman
Bejo, Mohd Hair
Ideris, Aini
Hamzah, Hairul Aini
Detection and distinguishing infectious bursal disease virus (IBDV) strains by molecular biology method
author_facet Omar, Abdul Rahman
Bejo, Mohd Hair
Ideris, Aini
Hamzah, Hairul Aini
author_sort Omar, Abdul Rahman
title Detection and distinguishing infectious bursal disease virus (IBDV) strains by molecular biology method
title_short Detection and distinguishing infectious bursal disease virus (IBDV) strains by molecular biology method
title_full Detection and distinguishing infectious bursal disease virus (IBDV) strains by molecular biology method
title_fullStr Detection and distinguishing infectious bursal disease virus (IBDV) strains by molecular biology method
title_full_unstemmed Detection and distinguishing infectious bursal disease virus (IBDV) strains by molecular biology method
title_sort detection and distinguishing infectious bursal disease virus (ibdv) strains by molecular biology method
publishDate 2010
url http://psasir.upm.edu.my/id/eprint/20452/
_version_ 1643827315941048320
score 13.18916

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