Changes in pH of legume cell suspension culture media containing aluminum.
When different levels of aluminum was added to the L-6 liquid media there was a marked decrease in pH values. The wide pH shift obtained indicated that pH adjustments prior to the addition of aluminum were essential in order to standardise the initial pH of all treatments at 4.0 ± 0.1. The pH ad...
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| Main Author: | |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
1990
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| Online Access: | http://psasir.upm.edu.my/id/eprint/18653/1/ID%2018653.pdf http://psasir.upm.edu.my/id/eprint/18653/ |
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| Summary: | When different levels of aluminum was added to the L-6 liquid
media there was a marked decrease in pH values. The wide pH
shift obtained indicated that pH adjustments prior to the
addition of aluminum were essential in order to standardise the
initial pH of all treatments at 4.0 ± 0.1. The pH adjustments
made to the L-6 liquid media prior to autoclaving for treatments
0, 50, 100, 200, 400, 600, and 800 uM Al were 4.4, 5.1, 5.6, 5.9,
6.1, 6.6, and 7.6, respectively. When the respective levels of
Al were added the pH of the media of each treatment was found to
be about 4.0 ± 0.1. Further examinations showed that the pH of
the culture media containing tepary bean cell suspensions and
whose initial pH was 4.5 increased gradually to about 5.0 within
12 days of culture. The pH of the culture media remained around
5.0 regardless of the initial pH values (4.5 and 5.3). However,
the pH appeared to be relatively constant at 4.0 ± 0.1 for five
days. This suggested that the cultures had to be transferred to
a fresh medium every 4-5 days to maintain the pH of the
media at 4.0 ± 0.1 and to maximise the toxic effects of aluminum. |
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