Two-step process for initial capture of plasmid DNA and partial removal of RNA using aqueous two-phase systems.

• Main Authors: Luechau, Frank, Ling, Tau Chuan, Lyddiatt, Andrew
• Format: Article
• Language: English; English
• Published: Elsevier BV 2010
• Online Access: http://psasir.upm.edu.my/id/eprint/17505/1/Two.pdf; http://psasir.upm.edu.my/id/eprint/17505/

In this paper, a two-step process for initial capture of plasmid DNA (pDNA) and partial removal of RNA using polyethylene glycol (PEG) and di-potassium hydrogen phosphate aqueous two-phase systems (ATPS) has been investigated. A Kühni-type ATPS extraction column was prepared with 50 ml (12% (w/w) PE...