The nucleocapsid protein of Newcastle disease virus promotes solubility of the VP2 hypervariable region of infectious bursal disease virus in Escherichia coli

• Main Authors: Saadun, Rafidah, Wen, Siang Tan, Omar, Abdul Rahman, Bejo, Mohd. Hair, Eshaghi, Majid, Yusoff, Khatijah
• Format: Article
• Language: English
• Published: Universiti Putra Malaysia Press 2008
• Online Access: http://psasir.upm.edu.my/id/eprint/17251/; http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2031%20(1)%20Feb.%202008/13%20page%2091-99.pdf

The hypervariable region (HVR) of VP2 protein of infectious bursal disease virus (IBDV) elicits neutralising antibodies, but it is highly hydrophobic and tends to form inclusion bodies when expressed in Escherichia coli. To improve its solubility, the VP2(HVR) was fused to the C-terminal end of Newc...