The development of an inhibitive determination method for zinc using a serine protease

• Main Authors: Abd. Shukor, Mohd. Yunus, Baharom, Nor Azlan, Masdor, Noor Azlina, Abdullah, Mohd Puad, Shamaan, Nor Aripin, Jamal, Jamia Azdina, Syed, Mohd Arif
• Format: Article
• Language: English
• Published: Triveni Enterprises 2009
• Online Access: http://psasir.upm.edu.my/id/eprint/17053/1/The%20development%20of%20an%20inhibitive%20determination%20method%20for%20zinc%20using%20a%20serine%20protease.pdf; http://psasir.upm.edu.my/id/eprint/17053/; http://www.jeb.co.in/index.php?page=issue_toc&issue=200901_jan09_spl

A new inhibitive heavy metals determination method using trypsin has been developed. The enzyme was assayed using the casein-Coomassie-dye-binding method. In the absence of inhibitors, casein was hydrolysed to completion and the Coomassie-dye was unable to stain the protein and the solution became b...