PCR-based DGGE and FISH analysis of methanogens in an anaerobic closed digester tank for treating palm oil mill effluent

• Main Authors: Tabatabaei, Meisam, Zakaria @ Mamat, Mohd Rafein, Abdul Rahim, Raha, Wright, Andre-Denis G., Shirai, Yoshihito, Abdullah, Norhani, Sakai, Kenji, Ikeno, Shinya, Mori, Masatsugu, Kazunori, Nakamura, Sulaiman, Alawi, Hassan, Mohd Ali
• Format: Article
• Language: English; English
• Published: Pontificia Universidad Católica de Valparaíso 2009
• Online Access: http://psasir.upm.edu.my/id/eprint/16061/1/16061.pdf; http://psasir.upm.edu.my/id/eprint/16061/; http://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/v12n3-4

16S ribosomal RNA (rRNA)-targeted fluorescent in situ hybridization combined with polymerase chain reaction (PCR)-cloning, light microscopy using Gram stains, scanning electron microscopy and denatured gradient gel electrophoresis were used to reveal the distribution of methanogens within an anaerob...