Occurrance and antibiotic resistance of Campylobacter jejuni and Campylobacter coli in retail broiler chicken

• Main Authors: M. R., Usha, Maarof, Fauziah, Robin, Tunung, Chai, Lay Ching, Cheah, Yoke Kqueen, Mohamad Ghazali, Farinazleen, Radu, Son
• Format: Article
• Language: English
• Published: Faculty of Food Science and Technology, Universiti Putra Malaysia 2010
• Online Access: http://psasir.upm.edu.my/id/eprint/15880/1/15880.pdf; http://psasir.upm.edu.my/id/eprint/15880/; http://www.ifrj.upm.edu.my/17%20%2802%29%202010/IFRJ-2010-247-255_Son_Malaysia%28S%29%5B1%5D.pdf

Broiler part samples (80 fresh and 80 chilled) were examined for the prevalence and numbers of C. jejuni and C. coli by employing most-probable-number (MPN) and polymerase chain reaction (PCR) techniques. The prevalence of the bacteria was high where C. jejuni was detected in 92.5% fresh and 53.8% chilled samples while C. coli in 80.0% fresh and 56.3% chilled. The number of these bacteria in the positive fresh and chilled samples was from 3 to more than 2400 MPN/g and from 3 to 290 MPN/g, respectively. Antibiotic resistance test (using Kirby-Bauer disc diffusion method) on 10 C. jejuni and 13 C. coli isolates toward ampicillin, tobramycin, enrofloxacin, ciprofloxacin, tetracycline, cephalothin, gentamicin and norfloxacin revealed high resistance toward all antibiotics (20.0% - 100.0%). All isolates were resistant to at least two antibiotics. This study highlights the potential of multidrug-resistant C. jejuni and C. coli transmission to humans through fresh and chilled broiler parts. Consecutive studies with bigger sample sizes and covering all over Malaysia are warranted in future.