Isolation and characterization of a Pseudomonas diesel-degrading strain from Antarctica

• Main Authors: Abd. Shukor, Mohd. Yunus, Ali Hassan, Nor Ayshah Alia, Jusoh, Arif Zaidi, Nagar, Perumal, Shamaan, Nor Aripin, Mac Cormack, Walter Patricio, Syed, Mohd Arif
• Format: Article
• Language: English
• Published: Triveni Enterprises 2009
• Online Access: http://psasir.upm.edu.my/id/eprint/15320/1/Isolation%20and%20characterization%20of%20a%20Pseudomonas%20diesel.pdf; http://psasir.upm.edu.my/id/eprint/15320/; http://www.jeb.co.in/index.php?page=issue_toc&issue=200901_jan09_spl

A diesel-degrading bacterium from Antarctica has been isolated. The isolate was tentatively identified as Pseudomonas sp. strain DRYJ3 based on partial 16SrDNA molecular phylogeny and Biolog® GN microplate panels and Microlog® database. Growth on diesel was supported optimally by ammonium sulphate, nitrate and nitrite. The bacterium grew optimally in between 10 and 15°C, pH 7.0 and 3.5% (v/v) diesel. The biodegradation of diesel oil by the strain increased in efficiency from the second to the sixth day of incubation from 1.4 to 18.8% before levelling off on the eighth day n-alkane oxidizing and aldehyde reductase activities were detected in the crude enzyme preparation suggesting the existence of terminal n-alkane oxidizing activity in this bacterium.