Detection of Escherichia coli O157:H7 by multiplex PCR and their characterization by plasmid profiling, antimicrobial resistance, RAPD and PFGE analyses

• Main Authors: Radu, Son, Ooi, Wai Ling, Rahmat Ali, Gulam Rusul, Abdul Karim, Mohamed Ismail, Nishibuchi, Mitsuaki
• 格式: Article
• 语言: English; English
• 出版: Elsevier 2001
• 在线阅读: http://psasir.upm.edu.my/id/eprint/15296/1/Detection%20of%20Escherichia%20coli%20O157H7%20by%20multiplex%20PCR%20and%20their%20characterization%20by%20plasmid%20profiling%2C%20antimicrobial%20resistance%2C%20RAPD%20and%20PFGE%20analyses.pdf; http://psasir.upm.edu.my/id/eprint/15296/7/1-s2.0-S016770120100269X-main.pdf; http://psasir.upm.edu.my/id/eprint/15296/; https://www.sciencedirect.com/science/article/pii/S016770120100269X?via%3Dihub#!

Twenty-five and three strains of Escherichia coli O157:H7 were identified from 25 tenderloin beef and three chicken meat burger samples, respectively. The bacteria were recovered using the immunomagnetic separation procedure followed by selective plating on sorbitol MacConkey agar and were identifie...