Molecular detection of Strongyloides ratti in faecal samples from wild rats in Serdang, Malaysia
Purpose: To detect Strongyloides ratti in faecal samples using conventional methods and to confirm the identification using a sensitive and specific method, namely, polymerase chain reaction (PCR). Methods: A PCR method targeting the small subunit of the rRNA gene was performed in this study for th...
Saved in:
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin
2015
|
Online Access: | http://psasir.upm.edu.my/id/eprint/15035/1/Molecular%20detection%20of%20Strongyloides%20ratti%20in%20faecal%20samples%20from%20wild%20rats%20in%20Serdang%2C%20Malaysia.pdf http://psasir.upm.edu.my/id/eprint/15035/ https://www.tjpr.org/home/abstract.php?id=178&aTitle=Molecular%20detection%20of%20Strongyloides%20ratti%20in%20faecal%20samples%20from%20wild%20rats%20in%20Serdang,%20Malaysia |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
my.upm.eprints.15035 |
---|---|
record_format |
eprints |
spelling |
my.upm.eprints.150352019-05-08T07:50:27Z http://psasir.upm.edu.my/id/eprint/15035/ Molecular detection of Strongyloides ratti in faecal samples from wild rats in Serdang, Malaysia Al-Zihiry, Khalid Jameel Kadhim Aliyu, Mahmuda Atshan, Salman Sahab Unyah, Ngah Zasmy Ibraheem, Zaid Osama Abd. Majid, Roslaini Awang Hamat, Rukman Abdullah, Wan Omar Purpose: To detect Strongyloides ratti in faecal samples using conventional methods and to confirm the identification using a sensitive and specific method, namely, polymerase chain reaction (PCR). Methods: A PCR method targeting the small subunit of the rRNA gene was performed in this study for the detection of DNA from Strongyloides ratti (an animal model of S. stercoralis) in faecal samples of wild Brown rats, Rattus norvegicus. Results: Strongyloides ratti was detected in 34.2 % of collected rats by different conventional techniques and confirmed by PCR. The essay presented 100 % sensitivity with Strongyloides universal primer. Conclusion: The findings of this study suggest that the application of PCR with universal primer is a very sensitive methodology to detect S. ratti in faecal material of wild rats infected even with very low parasite burden. Pharmacotherapy Group, Faculty of Pharmacy, University of Benin 2015 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/15035/1/Molecular%20detection%20of%20Strongyloides%20ratti%20in%20faecal%20samples%20from%20wild%20rats%20in%20Serdang%2C%20Malaysia.pdf Al-Zihiry, Khalid Jameel Kadhim and Aliyu, Mahmuda and Atshan, Salman Sahab and Unyah, Ngah Zasmy and Ibraheem, Zaid Osama and Abd. Majid, Roslaini and Awang Hamat, Rukman and Abdullah, Wan Omar (2015) Molecular detection of Strongyloides ratti in faecal samples from wild rats in Serdang, Malaysia. Tropical Journal of Pharmaceutical Research, 14 (7). pp. 1167-1173. ISSN 1596-5996; ESSN: 1596-9827 https://www.tjpr.org/home/abstract.php?id=178&aTitle=Molecular%20detection%20of%20Strongyloides%20ratti%20in%20faecal%20samples%20from%20wild%20rats%20in%20Serdang,%20Malaysia 10.4314/tjpr.v14i7.7 |
institution |
Universiti Putra Malaysia |
building |
UPM Library |
collection |
Institutional Repository |
continent |
Asia |
country |
Malaysia |
content_provider |
Universiti Putra Malaysia |
content_source |
UPM Institutional Repository |
url_provider |
http://psasir.upm.edu.my/ |
language |
English |
description |
Purpose: To detect Strongyloides ratti in faecal samples using conventional methods and to confirm the identification using a sensitive and specific method, namely, polymerase chain reaction (PCR).
Methods: A PCR method targeting the small subunit of the rRNA gene was performed in this study for the detection of DNA from Strongyloides ratti (an animal model of S. stercoralis) in faecal samples of wild Brown rats, Rattus norvegicus.
Results: Strongyloides ratti was detected in 34.2 % of collected rats by different conventional techniques and confirmed by PCR. The essay presented 100 % sensitivity with Strongyloides universal primer.
Conclusion: The findings of this study suggest that the application of PCR with universal primer is a very sensitive methodology to detect S. ratti in faecal material of wild rats infected even with very low parasite burden. |
format |
Article |
author |
Al-Zihiry, Khalid Jameel Kadhim Aliyu, Mahmuda Atshan, Salman Sahab Unyah, Ngah Zasmy Ibraheem, Zaid Osama Abd. Majid, Roslaini Awang Hamat, Rukman Abdullah, Wan Omar |
spellingShingle |
Al-Zihiry, Khalid Jameel Kadhim Aliyu, Mahmuda Atshan, Salman Sahab Unyah, Ngah Zasmy Ibraheem, Zaid Osama Abd. Majid, Roslaini Awang Hamat, Rukman Abdullah, Wan Omar Molecular detection of Strongyloides ratti in faecal samples from wild rats in Serdang, Malaysia |
author_facet |
Al-Zihiry, Khalid Jameel Kadhim Aliyu, Mahmuda Atshan, Salman Sahab Unyah, Ngah Zasmy Ibraheem, Zaid Osama Abd. Majid, Roslaini Awang Hamat, Rukman Abdullah, Wan Omar |
author_sort |
Al-Zihiry, Khalid Jameel Kadhim |
title |
Molecular detection of Strongyloides ratti in faecal samples from wild rats in Serdang, Malaysia |
title_short |
Molecular detection of Strongyloides ratti in faecal samples from wild rats in Serdang, Malaysia |
title_full |
Molecular detection of Strongyloides ratti in faecal samples from wild rats in Serdang, Malaysia |
title_fullStr |
Molecular detection of Strongyloides ratti in faecal samples from wild rats in Serdang, Malaysia |
title_full_unstemmed |
Molecular detection of Strongyloides ratti in faecal samples from wild rats in Serdang, Malaysia |
title_sort |
molecular detection of strongyloides ratti in faecal samples from wild rats in serdang, malaysia |
publisher |
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin |
publishDate |
2015 |
url |
http://psasir.upm.edu.my/id/eprint/15035/1/Molecular%20detection%20of%20Strongyloides%20ratti%20in%20faecal%20samples%20from%20wild%20rats%20in%20Serdang%2C%20Malaysia.pdf http://psasir.upm.edu.my/id/eprint/15035/ https://www.tjpr.org/home/abstract.php?id=178&aTitle=Molecular%20detection%20of%20Strongyloides%20ratti%20in%20faecal%20samples%20from%20wild%20rats%20in%20Serdang,%20Malaysia |
_version_ |
1643825813518286848 |
score |
13.160551 |