Establishing a culture system that supports in vitro expansion of adult microglia

• Main Authors: Subramaiam, Hemavathy, Badiei, Alireza, Ramasamy, Rajesh, Abdullah, Maha, Vidyadaran, Sharmili
• Format: Article
• Language: English
• Published: Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2010
• Online Access: http://psasir.upm.edu.my/id/eprint/14556/1/Establishing%20a%20culture%20system%20that%20supports%20in%20vitro%20expansion%20of%20adult%20microglia.pdf; http://psasir.upm.edu.my/id/eprint/14556/; http://www.medic.upm.edu.my/dokumen/FKUSK1_MJMHS_2010V06N2_OP04.pdf

Introduction: The vast majority of in vitro research on microglia are based on cells isolated from neonatal animals (3-5 days of age). Studying microglia of adults has been limited by the lack of a suitable culture system that supports their growth. In this study, we describe a protocol for growing...