Enhanced secretory production of hemolysin-mediated cyclodextrin-glucanotransferase in Escherichia coli by random mutagenesis of the ABC transporter system.

• Main Authors: Kheng, Oon Low, Mahadi, Nor Muhammad, Abdul Rahim, Raha, Rabu, Amir, Abu Bakar, Farah Diba, Abdul Murad, Abdul Munir, Md. Illias, Rosli
• Format: Article
• Language: English; English
• Published: Elsevier 2010
• Online Access: http://psasir.upm.edu.my/id/eprint/14512/1/Enhanced%20secretory%20production%20of%20hemolysin.pdf; http://psasir.upm.edu.my/id/eprint/14512/

The hemolysin transport system was found to mediate the release of cyclodextrin glucanotransferase (CGTase) into the extracellular medium when it was fused to the C-terminal 61 amino acids of HlyA (HlyAs(61)). To produce an improved-secretion variant, the hly components (hlyAs, hlyB and hlyD) were e...