Effects of plant growth regulators on callus induction from Cananga odorata flower petal explant

• Main Authors: Zain, Nurazah, Muse, Radzali, Ahmad, Syahida, Mahmood, Maziah
• Format: Article
• Language: English
• Published: Academic Journals 2009
• Online Access: http://psasir.upm.edu.my/id/eprint/14401/1/14401.pdf; http://psasir.upm.edu.my/id/eprint/14401/; http://www.academicjournals.org/journal/AJB/article-abstract/00AA34E8081

The Cananga odorata callus was initiated from petals of the C. odorata flowers on MS medium and B5 vitamins containing 30 g/L sugar and 3 g/L agar. The medium was also supplemented with different concentrations of 1-naphtalene acetic acid (NAA), and combinations of NAA with 6-benzylaminpurine(BAP) as the plant growth regulators. It was observed that the C. odorata callus could be induced on media containing the combination of 3 mg/L NAA and 0.5 mg/L BAP. High concentration of NAA gave rise to pale, whitish and friable callus after 1 - 2 weeks of culture. The optimum pH for the cell culture was about 5.7 and incubation at 25 ± 2°C, in totally dark condition.