Effect of Pluronic F-68, 5% CO2 atmosphere, HEPES, and antibiotic-antimycotic on suspension adapted 293 cells

• Main Authors: Negrete, Alejandro, Ling, Tau Chuan, Lyddiatt, Andrew
• Format: Article
• Language: English
• Published: Bentham Open 2008
• Online Access: http://psasir.upm.edu.my/id/eprint/14292/1/Effect%20of%20Pluronic%20F-68%2C%205%25%20CO2%20atmosphere%2C%20HEPES%2C%20and%20antibiotic-antimycotic%20on%20suspension%20adapted%20293%20cells.pdf; http://psasir.upm.edu.my/id/eprint/14292/; https://benthamopen.com/ABSTRACT/TOBIOTJ-2-229

The influence of different parameters upon cell culture of serum-free adapted 293 cells including the surfactant Pluronic F-68, carbon dioxide (5% CO2 atmosphere), buffer HEPES and antibiotic-antimycotic has been explored. A defined serum-free medium (SFM) formulated without human or animal origin c...