Development of multiplex-PCR for Genetically Modified Organism (GMO) detection targeting EPSPS and Cry1Ab genes in soy and maize samples
: The incidence of GMO is increasing worldwide therefore development of a reliable yet cost and time saving analytical method to detect GMO is important. This study aimed to develop a multiplex-PCR for GMO detection targeting Cry1Ab and EPSPS genes in soy and maize samples simultaneously, and se...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English English |
| Published: |
Faculty of Food Science and Technology, Universiti Putra Malaysia
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/14031/1/14031.pdf http://psasir.upm.edu.my/id/eprint/14031/7/Development%20of%20multiplex.pdf http://psasir.upm.edu.my/id/eprint/14031/ http://www.ifrj.upm.edu.my/volume-18-2011.html |
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| Summary: | : The incidence of GMO is increasing worldwide therefore development of a reliable yet cost and
time saving analytical method to detect GMO is important. This study aimed to develop a multiplex-PCR for
GMO detection targeting Cry1Ab and EPSPS genes in soy and maize samples simultaneously, and secondly
to obtain purified nucleic acids using CTAB DNA extraction method for conducting a GM specific analysis
on various types of food samples. The multiplex PCR was optimized to improve PCR performance and to
minimize failure. Out of 60 samples, 42 (70.0%) were found containing Cry1Ab or EPSPS genes, consisting of
11.9% of Roundup Ready Soya positive samples and 88.1% of Bt 176 Maize positive samples. Besides, 71.7%
samples yielded DNA concentration above 50 ng/µl; 66.7% samples were in the DNA purity range of 1.6 to
2.0 and 85.0% of the samples were amplifiable for the endogenous gene screening. The CTAB DNA extraction
method is effective for the DNA extraction from animal feeds, raw materials and processed foods. |
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