Development of an inhibitive enzyme assay for copper

• Main Authors: Abd. Shukor, Mohd. Yunus, Bakar, N. A., Othman, Ahmad Razi, Yunus, I., Shamaan, Nor Aripin, Syed, Mohd Arif
• Format: Article
• Language: English
• Published: Triveni Enterprises 2009
• Online Access: http://psasir.upm.edu.my/id/eprint/14003/1/Development%20of%20an%20inhibitive%20enzyme%20assay%20for%20copper.pdf; http://psasir.upm.edu.my/id/eprint/14003/; http://www.jeb.co.in/index.php?page=issue_toc&issue=200901_jan09_spl

In this work the development of an inhibitive assay for copper using the molybdenum-reducing enzyme assay is presented. The enzyme is assayed using 12-molybdophosphoric acid at pH 5.0 as an electron acceptor substrate and NADH as the electron donor substrate. The enzyme converts the yellowish soluti...