Development of a PCR assay for the detection of nifH and nifD genes in indigenous photosynthetic bacteria.

• Main Authors: Jee, Weng Tan, Kwai , Lin Thong, Arumugam, Nirmala Devi, Wai , Lian Cheah, Yih , Wei Lai, Kek, Heng Chua, Abdul Rahim, Raha, Sabaratnam, Vikineswary
• Format: Article
• Language: English; English
• Published: Elsevier 2009
• Online Access: http://psasir.upm.edu.my/id/eprint/13993/1/Development%20of%20a%20PCR%20assay%20for%20the%20detection%20of%20nifH%20and%20nifD%20genes%20in%20indigenous%20photosynthetic%20bacteria.pdf; http://psasir.upm.edu.my/id/eprint/13993/

Molybdenum (Mo) nitrogenases consist of two components: dinitrogenase reductase (encoded by nifH) and the dinitrogenase or MoFe protein (encoded by nifDK). Nitrogenase enzyme of photosynthetic bacteria is responsible for hydrogen production. Therefore, primers were designed for the nitrogenase gene...