Detection of virulence genes and enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) analysis among raw vegetables isolates of Campylobacter jejuni

• Main Authors: Abd. Mutalib, Sahilah, Tuan Soh, Tuan Suraya, Ismail, Noraida, Ariffin, Ahmad Azuhairi, Chai, Lay Ching, Radu, Son
• Format: Article
• Language: English
• Published: Faculty of Food Science and Technology, Universiti Putra Malaysia 2010
• Online Access: http://psasir.upm.edu.my/id/eprint/13940/1/13940.pdf; http://psasir.upm.edu.my/id/eprint/13940/; http://www.ifrj.upm.edu.my/17%20%2803%29%202010/IFRJ-2010-681-690%20Sahilah.pdf

A total of 20 (n=20) raw vegetable isolates of Campylobacter jejuni were examined for their virulence genes and genetic diversity by enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) analysis. All raw vegetable isolates of C. jejuni were encoded cadF and ceuE genes. None of the C. jejuni isolates was cdtA positive. Whereas, 12 (12/20) isolates were positive for cdtB and 6 (6/20) were positive for cdtC. All the 20 isolates of C. jejuni were subtyped and they produced 18 ERIC-PCR profiles namely E1 to E18. Dendrogram performed from cluster analysis showed that the 20 isolates were grouped into 2 major clusters, cluster I and II indicating the high level of local geographical genetic variation. The detection of virulence genes among C. jejuni strains isolated from raw vegetables raised concern on the threat of campylobacteriosis.