Detection and differentiation of velogenic and lentogenic Newcastle disease viruses using SYBR Green I real-time PCR with nucleocapsid gene-specific primers

• Main Authors: Tan, Sheau Wei, Ideris, Aini, Omar, Abdul Rahman, Mohd Yusoff, Khatijah, Bejo, Mohd Hair
• Format: Article
• Language: English
• Published: Elsevier 2009
• Online Access: http://psasir.upm.edu.my/id/eprint/13926/1/Detection%20and%20differentiation%20of%20velogenic%20and%20lentogenic%20Newcastle%20disease%20viruses%20using%20SYBR%20Green%20I%20real-time%20PCR%20with%20nucleocapsid%20gene-specific%20primers.pdf; http://psasir.upm.edu.my/id/eprint/13926/; https://www.sciencedirect.com/science/article/pii/S0166093409002389?via%3Dihub

SYBR Green I real-time PCR was developed for detection and differentiation of Newcastle disease virus (NDV). Primers based on the nucleocapsid (NP) gene were designed to detect specific sequence of velogenic strains and lentogenic/vaccine strains, respectively. The assay was developed and tested wit...