Chromosome analysis of walking catfish, Clarias spp.
Karyotyping is one of the useful tools for species identification, taxonomy, evolutionary and breeding selection. To date, no information on karyotyping of this fish in the country is documented. The aim of this study was to determine and differentiate the karyotypic structure and fundamental arm nu...
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Malaysian Society of Applied Biology
2009
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Online Access: | http://psasir.upm.edu.my/id/eprint/13593/ http://mabjournal.com/index.php?option=com_content&view=article&id=59:volume-381-june-2009&catid=37:current-view&Itemid=56 |
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my.upm.eprints.135932015-06-09T00:50:08Z http://psasir.upm.edu.my/id/eprint/13593/ Chromosome analysis of walking catfish, Clarias spp. Siraj, Siti Shapor Sukardi, Ratiah Cheah, Cheryl Phaik Imm Khew, Huai Mei Vellasamy, Shalini Panandam, Jothi Malar Daud, Siti Khalijah Karyotyping is one of the useful tools for species identification, taxonomy, evolutionary and breeding selection. To date, no information on karyotyping of this fish in the country is documented. The aim of this study was to determine and differentiate the karyotypic structure and fundamental arm number of the walking catfish; Clarias macrocephalus, Clarias batrachus and Clarias gariepinus. The fish samples ranging from 23–160gm in weight and 16–40cm in length were used to obtain the chromosomes by subjecting to a combination of colchicine and phytohaemagglutinin for arresting a mitotic division. The karyotype of each species was arranged according to size, type and structure such as metacentric (M), submetacentric (SM), subtelocentric (ST) and acrocentric/telocentric (A/T). The diploid chromosome numbers ranged from 2n equal to 54 (Clarias macrocephalus and Clarias batrachus) to 2n equal to 56 (Clarias gariepinus). Malaysian Society of Applied Biology 2009 Article PeerReviewed Siraj, Siti Shapor and Sukardi, Ratiah and Cheah, Cheryl Phaik Imm and Khew, Huai Mei and Vellasamy, Shalini and Panandam, Jothi Malar and Daud, Siti Khalijah (2009) Chromosome analysis of walking catfish, Clarias spp. Malaysian Applied Biology Journal, 38 (1). pp. 61-64. ISSN 0126-8643 http://mabjournal.com/index.php?option=com_content&view=article&id=59:volume-381-june-2009&catid=37:current-view&Itemid=56 |
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Karyotyping is one of the useful tools for species identification, taxonomy, evolutionary and breeding selection. To date, no information on karyotyping of this fish in the country is documented. The aim of this study was to determine and differentiate the karyotypic structure and fundamental arm number of the walking catfish; Clarias macrocephalus, Clarias batrachus and Clarias gariepinus. The fish samples ranging from 23–160gm in weight and 16–40cm in length were used to obtain the chromosomes by subjecting to a combination of colchicine and phytohaemagglutinin for arresting a mitotic division. The karyotype of each species was arranged according to size, type and structure such as metacentric (M), submetacentric (SM), subtelocentric (ST) and acrocentric/telocentric (A/T). The diploid chromosome numbers ranged from
2n equal to 54 (Clarias macrocephalus and Clarias batrachus) to 2n equal to 56 (Clarias gariepinus). |
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Siraj, Siti Shapor Sukardi, Ratiah Cheah, Cheryl Phaik Imm Khew, Huai Mei Vellasamy, Shalini Panandam, Jothi Malar Daud, Siti Khalijah |
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Siraj, Siti Shapor Sukardi, Ratiah Cheah, Cheryl Phaik Imm Khew, Huai Mei Vellasamy, Shalini Panandam, Jothi Malar Daud, Siti Khalijah Chromosome analysis of walking catfish, Clarias spp. |
author_facet |
Siraj, Siti Shapor Sukardi, Ratiah Cheah, Cheryl Phaik Imm Khew, Huai Mei Vellasamy, Shalini Panandam, Jothi Malar Daud, Siti Khalijah |
author_sort |
Siraj, Siti Shapor |
title |
Chromosome analysis of walking catfish, Clarias spp. |
title_short |
Chromosome analysis of walking catfish, Clarias spp. |
title_full |
Chromosome analysis of walking catfish, Clarias spp. |
title_fullStr |
Chromosome analysis of walking catfish, Clarias spp. |
title_full_unstemmed |
Chromosome analysis of walking catfish, Clarias spp. |
title_sort |
chromosome analysis of walking catfish, clarias spp. |
publisher |
Malaysian Society of Applied Biology |
publishDate |
2009 |
url |
http://psasir.upm.edu.my/id/eprint/13593/ http://mabjournal.com/index.php?option=com_content&view=article&id=59:volume-381-june-2009&catid=37:current-view&Itemid=56 |
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