Application of two dimensional thin layer chromatography pattern comparison for fingerprinting the active compounds in the leaves of vitex trifolia linn possessing anti-tracheospasmolytic activity.
We have developed one approach to fingerprint and estimate the active compounds in the leaves of Vitex trifolia Linn possessing anti-tracheospasmolytic assay using two-dimensional TLC pattern comparison. Based on the two-dimensional TLC pattern and the activity of the centrifugal partition chromatog...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Taylor & Francis
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/13246/1/Application%20of%20two%20dimensional%20thin%20layer%20chromatography%20pattern%20comparison%20for%20fingerprinting%20the%20active%20compounds%20in%20the%20leaves%20of%20Vitex%20trifolia%20Linn%20possessing%20anti.pdf http://psasir.upm.edu.my/id/eprint/13246/ |
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| Summary: | We have developed one approach to fingerprint and estimate the active compounds in the leaves of Vitex trifolia Linn possessing anti-tracheospasmolytic assay using two-dimensional TLC pattern comparison. Based on the two-dimensional TLC pattern and the activity of the centrifugal partition chromatography fractions, we concluded that the semi polar compounds were responsible for anti-tracheospasmolytic activity. The best non-polar/semi polar mobile phases for the two-dimensional TLC using silica gel as the stationary phase were chloroform/methanol (9/1) as the first mobile phase and ethyl acetate/chloroform/methanol (28/28/44) as the second mobile phase. |
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