Application of two dimensional thin layer chromatography pattern comparison for fingerprinting the active compounds in the leaves of Vitex trifolia Linn possessing anti-tracheospasmolytic activity.

• Main Authors: Khatib, Alfi, Hoek, Arie C., Selamat, Jinap, Sarker, Zaidul Islam, Jaswir, Irwandi, Verpoorte, Robert
• Format: Article
• Language: English; English
• Published: Taylor & Francis 2010
• Online Access: http://psasir.upm.edu.my/id/eprint/13245/1/Application%20of%20two%20dimensional%20thin%20layer%20chromatography%20pattern%20comparison%20for%20fingerprinting%20the%20active%20compounds%20in%20the%20leaves%20of%20Vitex%20trifolia%20Linn%20possessing%20anti.pdf; http://psasir.upm.edu.my/id/eprint/13245/

We have developed one approach to fingerprint and estimate the active compounds in the leaves of Vitex trifolia Linn possessing anti-tracheospasmolytic assay using two-dimensional TLC pattern comparison. Based on the two-dimensional TLC pattern and the activity of the centrifugal partition chromatography fractions, we concluded that the semi polar compounds were responsible for anti-tracheospasmolytic activity. The best non-polar/semi polar mobile phases for the two-dimensional TLC using silica gel as the stationary phase were chloroform/methanol (9/1) as the first mobile phase and ethyl acetate/chloroform/methanol (28/28/44) as the second mobile phase.