An inhibitive determination method for heavy metals using bromelain, a cysteine protease

• Main Authors: Abd. Shukor, Mohd. Yunus, Masdor, Noor Azlina, Baharom, Nor Azlan, Jamal, Jamia Azdina, Abdullah, Mohd Puad, Shamaan, Nor Aripin, Syed, Mohd Arif
• Format: Article
• Language: English
• Published: Humana Press 2008
• Online Access: http://psasir.upm.edu.my/id/eprint/13043/1/An%20inhibitive%20determination%20method%20for%20heavy%20metals%20using%20bromelain.pdf; http://psasir.upm.edu.my/id/eprint/13043/

A heavy-metal assay has been developed using bromelain, a protease. The enzyme is assayed using casein as a substrate with Coomassie dye to track completion of hydrolysis of casein. In the absence of inhibitors, casein is hydrolysed to completion, and the solution is brown. In the presence of metal...