A simplified multiplex PCR assay for fast and easy discrimination of globally distributed staphylococcal cassette chromosome mec types in meticillin-resistant Staphylococcus aureus

• Main Authors: Ghaznavi-Rad, Ehsanollah, Shamsudin, Mariana Nor, Sekawi, Zamberi, Belkum, Alex van, Neela, Vasantha Kumari
• Format: Article
• Language: English
• Published: Society for General Microbiology 2010
• Online Access: http://psasir.upm.edu.my/id/eprint/12859/1/A%20simplified%20multiplex%20PCR%20assay%20for%20fast%20and%20easy%20discrimination%20of%20globally%20distributed%20staphylococcal%20cassette%20chromosome%20mec%20types%20in%20meticillin.pdf; http://psasir.upm.edu.my/id/eprint/12859/; http://jmm.sgmjournals.org/content/59/10/1135.abstract

A multiplex PCR assay was developed for the identification of major types and subtypes of staphylococcal cassette chromosome mec (SCCmec) in meticillin-resistant Staphylococcus aureus (MRSA) strains. The method uses a novel 9 valent multiplex PCR plus two primer pairs for S. aureus identification an...