Development Of Microsatellite Markers And Genetic Diversity Assessment Of Kempas (Koompassia Malaccensis) In Peninsular Malaysia
A total of 24 novel microsatellite markers have been successfully isolated and characterised in an important tropical timber species of the family Leguminosae, Koompassia malaccensis, locally known as kempas. The microsatellite primers were designed from a genomic library enriched for dinucleotid...
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| Format: | Thesis |
| Language: | English English |
| Published: |
2009
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/12062/1/IB_2009_19_.pdf http://psasir.upm.edu.my/id/eprint/12062/ |
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| Summary: | A total of 24 novel microsatellite markers have been successfully isolated and
characterised in an important tropical timber species of the family Leguminosae,
Koompassia malaccensis, locally known as kempas. The microsatellite primers were
designed from a genomic library enriched for dinucleotide (CT) repeats and
subsequently screened on 24 samples from a natural population. In general, these
microsatellite markers are highly polymorphic (mean number of alleles per locus, Aa
= 6.84; average gene diversity, He = 0.692), with two loci found to deviate
significantly from Hardy-Weinberg equilibrium (p < 0.05). The utility of these
microsatellite markers were tested across 13 leguminous timber tree species and the
highest transferability was found with K. excelsa, the only species of the same genus
tested, followed by Dialium platysepalum of the same subtribe, Dialiinae. The
amplification success appeared to be inversely associated with the phylogenetic
distance, in particular up to the subtribal levels. Four of the microsatellite loci were
used to study the mating system of K. malaccensis, based on a fruiting season at the Semangkok Forest Reserve in year 2005. |
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